Determination of famotidine in low‐volume human plasma by normal‐phase liquid chromatography/tandem mass spectrometry
- 20 July 2001
- journal article
- research article
- Published by Wiley in Journal of Mass Spectrometry
- Vol. 36 (7), 736-741
- https://doi.org/10.1002/jms.176
Abstract
A rapid, sensitive and robust assay procedure using liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS) for the determination of famotidine in human plasma and urine is described. Famotidine and the internal standard were isolated from plasma samples by cation-exchange solid-phase extraction with benzenesulfonic acid (SCX) cartridges. The urine assay used direct injection of a diluted urine sample. The chromatographic separation was accomplished by using a BDS Hypersil silica column with a mobile phase of acetonitrile–water containing trifluoroacetic acid. The MS/MS detection of the analytes was set in the positive ionization mode using electrospray ionization for sample introduction. The analyte and internal standard precursor–product ion combinations were monitored in the multiple-reaction monitoring mode. Assay calibration curves were linear in the concentration range 0.5–500 ng ml−1 and 0.05–50 µg ml−1 in plasma and urine, respectively. For the plasma assay, a 100 µl sample aliquot was subjected to extraction. To perform the urine assay, a 50 µl sample aliquot was used. The intra-day relative standard deviations at all concentration levels were −1 in plasma and 0.05 µg ml−1 in urine. The methods were utilized to support clinical pharmacokinetic studies in infants aged 0–12 months. Copyright © 2001 John Wiley & Sons, Ltd.Keywords
This publication has 9 references indexed in Scilit:
- Separation and quantification of cholesterol and major phospholipid classes in human semen by high-performance liquid chromatography and light-scattering detectionJournal of Chromatography B: Biomedical Sciences and Applications, 2000
- Determination of chloroquine and desethylchloroquine in plasma and blood cells of Plasmodium vivax malaria cases using liquid chromatographyJournal of Pharmaceutical and Biomedical Analysis, 1999
- Pharmacokinetics and Pharmacodynamics of Famotidine in InfantsThe Journal of Clinical Pharmacology, 1998
- Determination of famotidine in human plasma by high performance liquid chromatography with column switchingJournal of Pharmaceutical and Biomedical Analysis, 1998
- Pharmacokinetics and Pharmacodynamics of Famotidine in ChildrenThe Journal of Clinical Pharmacology, 1996
- Drug monitoring of clomipramine and desmethylclomipramine in depressed patients using a new liquid chromatographic assayBiomedical Chromatography, 1993
- Suppression of gastric acid secretion by intravenous administration of famotidine in childrenThe Journal of Pediatrics, 1991
- FamotidineClinical Pharmacokinetics, 1990
- Analytical method for the quantification of famotidine, an H2-receptor blocker, in plasma and urineJournal of Chromatography B: Biomedical Sciences and Applications, 1985