Effects of Serial Passaging on the Adipogenic and Osteogenic Differentiation Potential of Adipose-Derived Human Mesenchymal Stem Cells
- 1 June 2007
- journal article
- Published by Mary Ann Liebert Inc in Tissue Engineering
- Vol. 13 (6), 1291-1298
- https://doi.org/10.1089/ten.2006.0275
Abstract
Adipose-derived human mesenchymal stem cells (hMSCs) will be more valuable for tissue engineering applications if they can be extensively subcultured without loss of phenotype and multilineage differentiation ability. This study examined the effects of serial passaging on growth rate, gene expression, and differentiation potential of adipose-derived hMSCs. Differentiation was assessed by analyzing changes in messenger RNA (mRNA) expression of osteogenic and adipogenic marker genes and by determining production of calcium deposits and lipid vacuoles. Cells cultured in osteogenic medium for 2 weeks upregulated expression of alkaline phosphatase mRNA relative to cells in growth medium, and deposited calcium. Calcium deposition decreased in cells from passages 4 to 6 but returned to levels near or above those of primary cells by passage 10. Cells cultured in adipogenic medium upregulated expression of lipoprotein lipase and peroxisome proliferator activated receptor-gamma mRNA relative to cells in growth medium, and formed lipid vacuoles at all passages. By passage 8, however, cells in adipogenic medium also deposited calcium. Growth rate was stable through passage 5, then decreased. The results of this study indicate that adipose-derived hMSCs are capable of both adipogenic and osteogenic differentiation through 10 passages (34 population doublings) but that osteogenic differentiation may start to dominate at later passages.Keywords
This publication has 27 references indexed in Scilit:
- Clonal analysis of the differentiation potential of human adipose‐derived adult stem cellsJournal of Cellular Physiology, 2005
- Mesenchymal Stem Cells from Human Bone Marrow and Adipose Tissue: Isolation, Characterization, and Differentiation PotentialitiesBulletin of Experimental Biology and Medicine, 2005
- Cell Surface and Transcriptional Characterization of Human Adipose‐Derived Adherent Stromal (hADAS) CellsThe International Journal of Cell Cloning, 2005
- Expression of Telomerase Extends the Lifespan and Enhances Osteogenic Differentiation of Adipose Tissue–Derived Stromal CellsThe International Journal of Cell Cloning, 2004
- Characterization and Expression Analysis of Mesenchymal Stem Cells from Human Bone Marrow and Adipose TissueCellular Physiology and Biochemistry, 2004
- Comparison of Multi-Lineage Cells from Human Adipose Tissue and Bone MarrowCells Tissues Organs, 2003
- Human Adipose Tissue Is a Source of Multipotent Stem CellsMolecular Biology of the Cell, 2002
- Surface protein characterization of human adipose tissue‐derived stromal cellsJournal of Cellular Physiology, 2001
- Propagation and senescence of human marrow stromal cells in culture: a simple colony‐forming assay identifies samples with the greatest potential to propagate and differentiateBritish Journal of Haematology, 1999
- Growth kinetics, self-renewal, and the osteogenic potential of purified human mesenchymal stem cells during extensive subcultivation and following cryopreservationJournal of Cellular Biochemistry, 1997