Oncogenic T‐antigen of JC virus is present frequently in human gastric cancers
Open Access
- 18 July 2006
- Vol. 107 (3), 481-488
- https://doi.org/10.1002/cncr.22028
Abstract
BACKGROUND. JC virus (JCV) is a polyomavirus that commonly infects humans and is the causative agent of progressive multifocal leukoencephalopathy in immune‐compromised patients. An association between JCV and human cancers long has been suspected, because this virus induces brain tumors in several animal models. The oncogenic potential of JCV is mediated by a transforming protein, the T‐antigen (T‐Ag), which is a multifunctional protein that transforms cells through interactions with various growth‐regulatory genes, including p53 and pRb, and by stabilizing β‐catenin. Previously, the laboratory at the authors' institution demonstrated that JCV is present frequently in the human gastrointestinal tract and may play a role in colorectal carcinogenesis. However, to date, no studies have determined whether JCV sequences are present specifically in gastric cancers. The current study was designed to investigate whether JCV sequences and expression are found in human gastric cancers. METHODS. DNA was extracted from 23 paraffin embedded and 14 frozen gastric cancer specimens. For the detection of JCV gene sequences, polymerase chain reaction amplifications were performed using gene‐specific primers for T‐Ag, VP‐1 (a JCV capsid gene), and the viral regulatory region (or transcriptional control region). Immunohistochemical staining was performed with an anti‐T‐Ag monoclonal antibody to detect protein expression. RESULTS. Twenty‐one of 37 gastric cancers (57%) harbored JCV T‐Ag sequences, and 13 of 37 gastric cancers (30%) contained VP‐1 sequences. T‐Ag sequences also were found in adjacent nonneoplastic mucosa. In addition, JCV regulatory region sequences were present frequently in gastric cancers and adjacent nonneoplastic mucosa. T‐Ag protein expression was found in 9 of 23 gastric cancers (39%), whereas no expression was observed in any of the nonneoplastic tissues. CONCLUSIONS. To the authors' knowledge, this is the first demonstration of the presence of JCV T‐Ag expression in human gastric cancers. These findings suggest a possible role for this polyomavirus in gastric carcinogenesis. Cancer 2006. © 2006 American Cancer Society.Keywords
This publication has 38 references indexed in Scilit:
- Detection of JC virus DNA sequences and expression of viral T antigen and agnoprotein in esophageal carcinomaCancer, 2005
- Potential Transmission of Human Polyomaviruses through the Gastrointestinal Tract after Exposure to Virions or Viral DNAJournal of Virology, 2001
- The epidemiology and prevention of hepatocellular carcinomaSeminars in Oncology, 2001
- Mad-1 Is the Exclusive JC Virus Strain Present in the Human Colon, and Its Transcriptional Control Region Has a Deleted 98-Base-Pair Sequence in Colon Cancer TissuesJournal of Virology, 2001
- SV40 and the pathogenesis of mesotheliomaSeminars in Cancer Biology, 2001
- In situmRNA Hybridization Technique for Analysis of Human Telomerase RNA in Gastric Precancerous and Cancerous LesionsJapanese Journal of Cancer Research, 1998
- Establishment and characterization of a carrier cell culture producing high titres of polyoma JC virusJournal of Medical Virology, 1995
- Cytogenetic findings in eleven gastric carcinomasCancer Genetics and Cytogenetics, 1993
- Molecular mechanism of stomach carcinogenesisZeitschrift für Krebsforschung und Klinische Onkologie, 1993
- Human Papovavirus (JC): Induction of Brain Tumors in HamstersScience, 1973