Oxidative DNA damage induced by simultaneous generation of nitric oxide and superoxide
- 28 August 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 371 (1), 86-88
- https://doi.org/10.1016/0014-5793(95)00873-8
Abstract
Incubation of calf thymus DNA with 3-morpholinosydnonimine (SIN-1), which simultaneously generates nitric oxide (NO) and superoxide (O2−), induced a significant increase of 8-hydroxydeoxyguanosine (8-OH-dG). Peroxynitrite also increased 8-OH-dG in calf thymus DNA. Addition of free hydroxyl radical (•OH) scavengers inhibited the increase of 8-OH-dG by SIN-1 or peroxynitrite. Incubation of 32P-labeled DNA fragment with SIN-1 or peroxynitrite caused DNA cleavage at every nucleotide with a little dominance at guanine residues. The results suggest that NO reacts with O2− to form peroxynitrite and the peroxynitrite induces oxidative DNA damage through an active intermediate of which reactivity is similar to •OHThis publication has 18 references indexed in Scilit:
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