Budding YeastSSD1-VRegulates Transcript Levels of Many Longevity Genes and Extends Chronological Life Span in Purified Quiescent Cells
- 1 September 2009
- journal article
- Published by American Society for Cell Biology (ASCB) in Molecular Biology of the Cell
- Vol. 20 (17), 3851-3864
- https://doi.org/10.1091/mbc.e09-04-0347
Abstract
Ssd1 is an RNA-binding protein that affects literally hundreds of different processes and is polymorphic in both wild and lab yeast strains. We have used transcript microarrays to compare mRNA levels in an isogenic pair of mutant (ssd1-d) and wild-type (SSD1-V) cells across the cell cycle. We find that 15% of transcripts are differentially expressed, but there is no correlation with those mRNAs bound by Ssd1. About 20% of cell cycle regulated transcripts are affected, and most show sharper amplitudes of oscillation in SSD1-V cells. Many transcripts whose gene products influence longevity are also affected, the largest class of which is involved in translation. Ribosomal protein mRNAs are globally down-regulated by SSD1-V. SSD1-V has been shown to increase replicative life span¤ and we show that SSD1-V also dramatically increases chronological life span (CLS). Using a new assay of CLS in pure populations of quiescent prototrophs, we find that the CLS for SSD1-V cells is twice that of ssd1-d cells.Keywords
This publication has 93 references indexed in Scilit:
- Autophagy and amino acid homeostasis are required for chronological longevity in Saccharomyces cerevisiaeAging Cell, 2009
- Influence of genotype and nutrition on survival and metabolism of starving yeastProceedings of the National Academy of Sciences of the United States of America, 2008
- The Atg1 Kinase Complex Is Involved in the Regulation of Protein Recruitment to Initiate Sequestering Vesicle Formation for Nonspecific Autophagy inSaccharomyces cerevisiaeMolecular Biology of the Cell, 2008
- Gene Ontology annotations at SGD: new data sources and annotation methodsNucleic Acids Research, 2007
- The highways and byways of mRNA decayNature Reviews Molecular Cell Biology, 2007
- Conservation of the metabolomic response to starvation across two divergent microbesProceedings of the National Academy of Sciences of the United States of America, 2006
- The Forkhead transcription factor Hcm1 regulates chromosome segregation genes and fills the S-phase gap in the transcriptional circuitry of the cell cycleGenes & Development, 2006
- Isolation of quiescent and nonquiescent cells from yeast stationary-phase culturesThe Journal of cell biology, 2006
- Integration of chemical-genetic and genetic interaction data links bioactive compounds to cellular target pathwaysNature Biotechnology, 2003
- A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistanceMolecular Genetics and Genomics, 1984