Preexisting and de novo humoral immunity to SARS-CoV-2 in humans
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Open Access
- 11 December 2020
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 370 (6522), 1339-1343
- https://doi.org/10.1126/science.abe1107
Abstract
Zoonotic introduction of novel coronaviruses may encounter preexisting immunity in humans. Using diverse assays for antibodies recognizing SARS-CoV-2 proteins, we detect preexisting humoral immunity. SARS-CoV-2 spike glycoprotein (S)-reactive antibodies were detectable by a flow cytometry-based method in SARS-CoV-2-uninfected individuals and were particularly prevalent in children and adolescents. They were predominantly of the IgG class and targeted the S2 subunit. By contrast, SARS-CoV-2 infection induced higher titers of SARS-CoV-2 S-reactive IgG antibodies, targeting both the S1 and S2 subunits, and concomitant IgM and IgA antibodies, lasting throughout the observation period. Notably, SARS-CoV-2-uninfected donor sera exhibited specific neutralizing activity against SARS-CoV-2 and SARS-CoV-2 S pseudotypes. Distinguishing preexisting and de novo immunity will be critical for our understanding of susceptibility to and the natural course of SARS-CoV-2 infection.Keywords
This publication has 43 references indexed in Scilit:
- Immunogenicity and structures of a rationally designed prefusion MERS-CoV spike antigenProceedings of the National Academy of Sciences of the United States of America, 2017
- Optimisation of a micro‐neutralisation assay and its application in antigenic characterisation of influenza virusesInfluenza and Other Respiratory Viruses, 2015
- Coronaviruses Induce Entry-Independent, Continuous MacropinocytosismBio, 2014
- Reconstitution of the Ancestral Glycoprotein of Human Endogenous Retrovirus K and Modulation of Its Functional Activity by Truncation of the Cytoplasmic DomainJournal of Virology, 2009
- Development of a Nucleocapsid-Based Human Coronavirus Immunoassay and Estimates of Individuals Exposed to Coronavirus in a U.S. Metropolitan PopulationClinical and Vaccine Immunology, 2008
- Human Coronavirus NL63 and 229E Seroconversion in ChildrenJournal of Clinical Microbiology, 2008
- Human Monoclonal Antibody Combination against SARS Coronavirus: Synergy and Coverage of Escape MutantsPLoS Medicine, 2006
- MHVR-Independent Cell-Cell Spread of Mouse Hepatitis Virus Infection Requires Neutral pH FusionAdvances in Experimental Medicine and Biology, 1995
- The time course of the immune response to experimental coronavirus infection of manEpidemiology and Infection, 1990