Quantifying Transfer Rates of Salmonella and Escherichia coli O157:H7 between Fresh-Cut Produce and Common Kitchen Surfaces
- 1 September 2013
- journal article
- Published by Elsevier BV in Journal of Food Protection
- Vol. 76 (9), 1530-1538
- https://doi.org/10.4315/0362-028x.jfp-13-098
Abstract
Cross-contamination between foods and surfaces in food processing environments and home kitchens may play a significant role in foodborne disease transmission. This study quantifies the cross-contamination rates between a variety of fresh-cut produce and common kitchen surfaces (ceramic, stainless steel, glass, and plastic) using scenarios that differ by cross-contamination direction, surface type, produce type, and drying time/moisture level. A five-strain cocktail of rifampin-resistant Salmonella was used in transfer scenarios involving celery, carrot, and watermelon, and a five-strain cocktail of rifampin-resistant Escherichia coli O157:H7 was used in transfer scenarios involving lettuce. Produce or surface coupons were placed in buffer-filled filter bags and homogenized or massaged, respectively, to recover cells. The resulting solutions were serially diluted in 0.1% peptone and surface plated onto tryptic soy agar with 80 μg/ml rifampin and bismuth sulfite agar with 80 μg/ml rifampin for Salmonella or sorbitol MacConkey agar with 80 μg/ml rifampin for E. coli O157:H7. When the food contact surface was freshly inoculated, a high amount (>90%) of the inoculum was almost always transferred to the cut produce item. If the inoculated food contact surfaces were allowed to dry for 1 h, median transfer was generally >90% for carrots and watermelon but ranged from <1 to ∼70% for celery and lettuce. Freshly inoculated celery or lettuce transferred more bacteria (<2 to ∼25% of the inoculum) compared with freshly inoculated carrots or watermelon (approximately <1 to 8%). After 1 h of drying, the rate of transfer from inoculated celery, carrot, and lettuce was <0.01 to ∼5% and <1 to ∼5% for watermelon. Surface moisture and direction of transfer have the greatest influence on microbial transfer rates.Keywords
Funding Information
- U.S. Food and Drug Administration (1R01FD003672-01)
This publication has 29 references indexed in Scilit:
- Attribution of Foodborne Illnesses, Hospitalizations, and Deaths to Food Commodities by using Outbreak Data, United States, 1998–2008Emerging Infectious Diseases, 2013
- Yersinia pseudotuberculosisO:1 Traced to Raw Carrots, FinlandEmerging Infectious Diseases, 2008
- Cross-contamination in the kitchen: estimation of transfer rates for cutting boards, hands and knivesJournal of Applied Microbiology, 2008
- Curli Biogenesis and FunctionAnnual Review of Microbiology, 2006
- Thin Aggregative Fimbriae and Cellulose Enhance Long-Term Survival and Persistence ofSalmonellaJournal of Bacteriology, 2006
- Reducing Salmonella on cantaloupes and honeydew melons using wash practices applicable to postharvest handling, foodservice, and consumer preparationInternational Journal of Food Microbiology, 2005
- Biofilm Formation by Escherichia coli O157:H7 on Stainless Steel: Effect of Exopolysaccharide and Curli Production on Its Resistance to ChlorineApplied and Environmental Microbiology, 2005
- Influence of the incubation temperature, atmosphere and dynamic conditions on biofilm formation by Salmonella spp.Food Microbiology, 2003
- Reduction of faecal coliform, coliform and heterotrophic plate count bacteria in the household kitchen and bathroom by disinfection with hypochlorite cleanersJournal of Applied Microbiology, 1998
- The survival and transfer of microbial contamination via cloths, hands and utensilsJournal of Applied Bacteriology, 1990