Comparison of platelet aggregation using light transmission and multiple electrode aggregometry in Glanzmann thrombasthenia
- 1 January 2009
- journal article
- research article
- Published by Taylor & Francis Ltd in Platelets
- Vol. 20 (5), 297-301
- https://doi.org/10.1080/09537100903006246
Abstract
We examined platelet aggregation in platelet-rich plasma (PRP) and in whole blood in nine patients with Thrombasthenia Glanzmann (TG). In PRP, aggregation was measured by monitoring the changes in light absorbance that occurred in response to adenosine 5-diphosphate (ADP), collagen and ristocetin. To measure platelet aggregation in whole blood, we used multiple electrode Impedance aggregometry using the same aggregating agents. In PRP, the patient's platelets showed defective aggregation in response to ADP, collagen, epinephrine and partially to ristocetin in all patients. In whole blood, platelet aggregation in response to the same aggregating agents showed similar response and appeared to be very similar to that which occurred in PRP. Whole blood impedance aggregometry seems to give similar results to PRP light transmission aggregometry in patients with TG. Multiple electrode aggregometry (MEA) is faster and more convenient to use in these patients.Keywords
This publication has 25 references indexed in Scilit:
- Novel mutations in GP IIb gene in Glanzmann's thrombasthenia from IndiaPlatelets, 2009
- Inherited traits affecting platelet functionBlood Reviews, 2008
- Current Options in Platelet Function TestingThe American Journal of Cardiology, 2006
- Glanzmann thrombastheniaOrphanet Journal of Rare Diseases, 2006
- Acquired thrombasthenia due to GPIIbIIIa platelet autoantibodies in a 4‐yr‐old childEuropean Journal of Haematology, 2005
- Application of Flow Cytometry to Platelet DisordersSeminars in Thrombosis and Hemostasis, 2004
- Glanzmann's thrombasthenia: updatedPlatelets, 2002
- Platelet Glycoprotein IIb/IIIa Receptors and Glanzmann’s ThrombastheniaArteriosclerosis, Thrombosis, and Vascular Biology, 2000
- Rapid and Simple Platelet Function Assay to Assess Glycoprotein IIb/IIIa Receptor BlockadeCirculation, 1997
- Delivery of infants with Glanzmann thrombasthenia and subsequent blood transfusion requirements: A follow‐up of 39 patientsAmerican Journal of Hematology, 1992