Associations between Antimicrobial Resistance Genes in Fecal Generic Escherichia coli Isolates from Cow-Calf Herds in Western Canada
- 15 June 2008
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 74 (12), 3658-3666
- https://doi.org/10.1128/aem.02505-07
Abstract
The objective of this study was to examine associations among the genetic determinants of antimicrobial resistance (AMR) in 207 fecal generic Escherichia coli isolates obtained from 77 cow-calf herds in western Canada. Twenty-three resistance genes corresponding to six different antimicrobial families were assessed using DNA hybridization and PCR. The most common resistance genes in the study sample (207 isolates) were sul2 (48.3%), tet (B) (45.4%), and ant(3 ″ )-Ia ( aadA1 ) (19.3%). Several statistically significant associations between the examined resistance genes were detected. The strongest associations observed were those between genes for resistance to chloramphenicol ( catI ) and trimethoprim ( dhfrI ) (odds ratio [OR] = 214; P = 0.0001), sulfonamide ( sul1 ) and chloramphenicol ( catI ) (OR = 96.9; P = 0.0001), streptomycin [ ant(3 ″ )-Ia ( aadA1 )] and trimethoprim ( dhfrI ) (OR = 96.2; P = 0.0001), sulfonamide ( sul1 ) and streptomycin [ ant(3 ″ )-Ia ( aadA1 )] (OR = 79.3; P = 0.0001), and tetracycline [ tet (B)] and sulfonamides ( sul2 ) (OR = 25.7; P = 0.0001). At least one of the resistance genes corresponding to each nonaminoglycoside family of antimicrobials examined in this study was associated with the two aminoglycoside resistance genes ant(3 ″ )-Ia ( aadA1 ) and aph(3 ′)- Ia . The multiple, strong associations between genes and the diverse nature of the associations described in this study demonstrate the complexity of resistance gene selection in cow-calf herds and should be considered in the planning of AMR control practices for cow-calf operations.Keywords
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