Small RNA asymmetry in RNAi: Function in RISC assembly and gene regulation
- 20 September 2005
- journal article
- review article
- Published by Wiley in FEBS Letters
- Vol. 579 (26), 5850-5857
- https://doi.org/10.1016/j.febslet.2005.08.071
Abstract
RNAi is a conserved gene-specific regulatory mechanism, which silences target gene expression transcriptionally and post-transcriptionally. The RNAi machinery converts the sequence specific information of a long double stranded RNAs (dsRNAs) into small 21–22 nt long dsRNAs (siRNAs, miRNAs) which assemble into an effector complex, the RNA induced silencing complex (RISC). RISC assembly is asymmetric; one strand of an siRNA or a miRNA preferentially incorporates into the RNA–protein complex. Here, I review the rules of the asymmetric RISC formation and discuss their possible regulatory function in several steps in RNAiThis publication has 92 references indexed in Scilit:
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