Effect of CPP-ACP paste on tooth mineralization: an FE-SEM study
Open Access
- 1 January 2007
- journal article
- Published by Nihon University School of Dentistry in Journal of Oral Science
- Vol. 49 (2), 115-120
- https://doi.org/10.2334/josnusd.49.115
Abstract
Milk and milk products, such as cheese, have been shown to exhibit anticariogenic properties in human and animal models. CPP-ACP shows an anti-caries effect by suppressing demineralization, enhancing remineralization, or possibly a combination of both. The purpose of this study was to evaluate the effect of CPP-ACP paste on demineralization by observing the treated tooth surface using an FE-SEM. The specimens were prepared by cutting enamel and dentin of bovine teeth into blocks. A few specimens were stored in 0.1 M lactic acid buffer solution for 10 min and then in artificial saliva (negative control). The remaining specimens were stored in a 10 times-diluted solution of CPP-ACP paste or a placebo paste containing no CPP-ACP for 10 min, followed by 10 min immersion in a demineralizing solution (pH = 4.75, Ca) twice a day before storage in artificial saliva. After treatment of the specimens for 3, 7, 21 and 28 days, they were fixed in 2.5% glutaraldehyde in cacodylate buffer solution, dehydrated in ascending grades of tert-butyl alcohol, and then transferred to a critical-point dryer. The surfaces were coated with a thin film of Au in a vacuum evaporator, and were observed under field emission-scanning electron microscopy (FE-SEM). The SEM observations revealed different morphological features brought about by the various storage conditions. Demineralization of the enamel and dentin surfaces was more pronounced with the longer test period in the control and negative control specimens. On the other hand, enamel and dentin specimens treated with CPP-ACP paste revealed slight changes in their morphological features. From the morphological observations of the enamel and dentin surfaces, it could be considered that the CPP-ACP paste might prevent demineralization of the tooth structure. (J. Oral Sci. 49, 115-120, 2007)Keywords
This publication has 21 references indexed in Scilit:
- A Critical Review of Non-carious Cervical (Wear) Lesions and the Role of Abfraction, Erosion, and AbrasionJournal of Dental Research, 2006
- The prevalence of non‐carious cervical lesions in permanent dentitionJournal of Oral Rehabilitation, 2004
- Development of low erosive carbonated fruit drinks 2. Evaluation of an experimental carbonated blackcurrant drink compared to a conventional carbonated drinkJournal of Dentistry, 2003
- Dental erosion – changing prevalence? A review of British national childrens’ surveysInternational Journal of Paediatric Dentistry, 2003
- Cervical tooth wear and sensitivity: erosion, softening and rehardening of dentine; effects of pH, time and ultrasonicationJournal of Clinical Periodontology, 2002
- A comparison of the erosive potential of different beverages in primary and permanent teeth using an in vitro modelEuropean Journal of Oral Sciences, 2000
- Minimal intervention dentistry — a reviewInternational Dental Journal, 2000
- The aetiology of the non-carious cervical lesionInternational Dental Journal, 1999
- The caries decline: a review of reviewsEuropean Journal of Oral Sciences, 1996
- Effect of milk on caries incidence and bacterial composition of dental plaque in the ratArchives of Oral Biology, 1981