Heparan Sulfate Mediates Infection of High-Neurovirulence Theiler's Viruses
Open Access
- 15 August 2002
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 76 (16), 8400-8407
- https://doi.org/10.1128/jvi.76.16.8400-8407.2002
Abstract
The mechanisms by which Theiler's murine encephalomyelitis virus (TMEV) binds and enters host cells and the molecules involved are not completely understood. In this study, we demonstrate that the high-neurovirulence TMEV GDVII virus uses the glycosaminoglycan heparan sulfate (HS) as an attachment factor that is required for efficient infection. Studies based on soluble HS-mediated inhibition of attachment and infection, removal of HS with specific enzymes, and blocking with anti-HS antibodies establish that HS mediates GDVII virus entry into mammalian cells. Data from defined proteoglycan-deficient Chinese hamster ovary mutant cells further support the role of HS in GDVII infection and indicate that the extent of sulfation is critical for infection. Neuraminidase treatment of proteoglycan-deficient cells restores permissiveness to GDVII virus, indicating that sialic acid hinders direct access of virus to the protein entry receptor. A model of the potential steps in GDVII virus entry into mammalian cells involving HS is proposed.Keywords
This publication has 33 references indexed in Scilit:
- Integrin αvβ1 Is a Receptor for Foot-and-Mouth Disease VirusJournal of Virology, 2002
- UDP-Galactose Transporter Is Required for Theiler's Virus Entry into Mammalian CellsVirology, 2001
- Echoviruses Bind Heparan Sulfate at the Cell SurfaceJournal of Virology, 2001
- Role of the Cytoplasmic Domain of the β-Subunit of Integrin αvβ6 in Infection by Foot-and-Mouth Disease VirusJournal of Virology, 2001
- Functions of Cell Surface Heparan Sulfate ProteoglycansAnnual Review of Biochemistry, 1999
- Isolation of a Common Receptor for Coxsackie B Viruses and Adenoviruses 2 and 5Science, 1997
- The Structure of a Highly Virulent Theiler's Murine Encephalomyelitis Virus (GDVII) and Implications for Determinants of Viral PersistenceVirology, 1996
- Molecular modeling of protein-glycosaminoglycan interactions.Arteriosclerosis: An Official Journal of the American Heart Association, Inc., 1989
- Rapid colorimetric assay for cell growth and survivalJournal of Immunological Methods, 1986
- Demyelinative myelopathy in mice induced by the DA virusJournal of the Neurological Sciences, 1976