M13 single-strand purification using a biotinylated probe and streptavidin coated magnetic beads
- 1 January 1992
- journal article
- research article
- Published by Taylor & Francis Ltd in DNA Sequence
- Vol. 3 (2), 65-69
- https://doi.org/10.3109/10425179209033998
Abstract
A new method for M13 single-strand purification has been developed and tested. The procedure gives sufficient DNA for one set of thermally cycled sequencing reactions when analysed on the Applied Biosystems 373A fluorescent DNA sequencer. For this report, 250 random M13 subclones with 1-2 Kb inserts were grown and prepared using this method.Keywords
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