Acetazolamide-based fungal chitinase inhibitors
Open Access
- 8 October 2010
- journal article
- Published by Elsevier BV in Bioorganic & Medicinal Chemistry
- Vol. 18 (23), 8334-8340
- https://doi.org/10.1016/j.bmc.2010.09.062
Abstract
Chitin is an essential structural component of the fungal cell wall. Chitinases are thought to be important for fungal cell wall remodelling, and inhibition of these enzymes has been proposed as a potential strategy for development of novel anti-fungals. The fungal pathogen Aspergillus fumigatus possesses two distinct multi-gene chitinase families. Here we explore acetazolamide as a chemical scaffold for the inhibition of an A. fumigatus ‘plant-type’ chitinase. A co-crystal structure of AfChiA1 with acetazolamide was used to guide synthesis and screening of acetazolamide analogues that yielded SAR in agreement with these structural data. Although acetazolamide and its analogues are weak inhibitors of the enzyme, they have a high ligand efficiency and as such are interesting leads for future inhibitor development.Keywords
Funding Information
- Wellcome Trust Senior Research Fellowship (081745)
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