Synthesis of Modified Guanidine-Based Polymers and their Antimicrobial Activities Revealed by AFM and CLSM
- 14 April 2011
- journal article
- Published by American Chemical Society (ACS) in ACS Applied Materials & Interfaces
- Vol. 3 (6), 1895-1901
- https://doi.org/10.1021/am200094u
Abstract
Modified guanidine-based polymers with chain extension were synthesized by condensation and cross-linking polymerizations in an attempt to increase molecular weight and charge density of the antimicrobial polymers. The antimicrobial activity and the corresponding mechanisms were investigated by several approaches. The results indicated that the antimicrobial activities of the modified guanidine-based polymer, based on the minimum inhibition concentration (MIC) against E.coli, varied with alkyl monomer ratios. UV absorption at 260 nm further quantified the amount of intracellular components leaked into bacteria suspension. The UV absorption measurements were also used to monitor inhibition processes dynamically. It was found that the modified guanidine-based polymer inhibited the growth of bacteria by causing membrane compromised and intracellular leaked. Dual fluorescent dyes were used to stain all bacteria including the dead ones, which enabled us to utilize CLSM to visualize the viability of bacteria in the presence of various modified guanidine-based polymers without causing any damage. The morphologies of bacteria untreated and treated with modified guanidine-based polymer were observed using an atomic force microscope (AFM), which further demonstrated the damage of E.coli membrane and the leakage of intracellular component induced by the modified guanidine-based polymers.Keywords
This publication has 32 references indexed in Scilit:
- New Approach for Imparting Antimicrobial Properties for Polyamide and Wool Containing FabricsPolymer-Plastics Technology and Engineering, 2007
- Antimicrobial-modified sulfite pulps prepared by in situ copolymerizationCarbohydrate Polymers, 2007
- The Chemistry and Applications of Antimicrobial Polymers: A State-of-the-Art ReviewBiomacromolecules, 2007
- Grafting of Poly[2-(tert-butylamino)ethyl methacrylate] onto Polypropylene by Reactive Blending and Antibacterial Activity of the CopolymerBiomacromolecules, 2007
- Studies on the preparation and antibacterial properties of quaternized polyethyleneimineJournal of Biomaterials Science, Polymer Edition, 2007
- Feasibility evaluation of chitosan coatings on polyethylene tubing for biliary stent applicationsJournal of Applied Polymer Science, 2005
- Membrane-based processes performed with use of chitin/chitosan materialsSeparation and Purification Technology, 2005
- Application of a fiber-reactive chitosan derivative to cotton fabric as an antimicrobial textile finishCarbohydrate Polymers, 2004
- Quaternary Ammonium Functionalized Poly(propylene imine) Dendrimers as Effective Antimicrobials: Structure−Activity StudiesBiomacromolecules, 2000
- Mechanisms of action of antibacterial biocidesInternational Biodeterioration & Biodegradation, 1995