Conserved Processes and Lineage-Specific Proteins in Fungal Cell Wall Evolution
- 1 December 2007
- journal article
- research article
- Published by American Society for Microbiology in Eukaryotic Cell
- Vol. 6 (12), 2269-2277
- https://doi.org/10.1128/ec.00044-07
Abstract
The cell wall is a defining organelle that differentiates fungi from its sister clades in the opisthokont superkingdom. With a sensitive technique to align low-complexity protein sequences, we have identified 187 cell wall-related proteins in Saccharomyces cerevisiae and determined the presence or absence of homologs in 17 other fungal genomes. There were both conserved and lineage-specific cell wall proteins, and the degree of conservation was strongly correlated with protein function. Some functional classes were poorly conserved and lineage specific: adhesins, structural wall glycoprotein components, and unannotated open reading frames. These proteins are primarily those that are constituents of the walls themselves. On the other hand, glycosyl hydrolases and transferases, proteases, lipases, proteins in the glycosyl phosphatidyl-inositol-protein synthesis pathway, and chaperones were strongly conserved. Many of these proteins are also conserved in other eukaryotes and are associated with wall synthesis in plants. This gene conservation, along with known similarities in wall architecture, implies that the basic architecture of fungal walls is ancestral to the divergence of the ascomycetes and basidiomycetes. The contrasting lineage specificity of wall resident proteins implies diversification. Therefore, fungal cell walls consist of rapidly diversifying proteins that are assembled by the products of an ancestral and conserved set of genes.Keywords
This publication has 76 references indexed in Scilit:
- Between-Species Analysis of Short-Repeat Modules in Cell Wall and Sex-Related Hydroxyproline-Rich Glycoproteins ofChlamydomonasPlant Physiology, 2007
- A Biochemical Guide to Yeast Adhesins: Glycoproteins for Social and Antisocial OccasionsMicrobiology and Molecular Biology Reviews, 2007
- Protein Glycosylation, Conserved from Yeast to Man: A Model Organism Helps Elucidate Congenital Human DiseasesAngewandte Chemie, 2006
- Cell Wall Assembly in Saccharomyces cerevisiaeMicrobiology and Molecular Biology Reviews, 2006
- Cell evolution and Earth history: stasis and revolutionPhilosophical Transactions B, 2006
- Composition-Modified Matrices Improve Identification of Homologs of Saccharomyces cerevisiae Low-Complexity GlycoproteinsEukaryotic Cell, 2006
- Improved Prediction of Signal Peptides: SignalP 3.0Journal of Molecular Biology, 2004
- A sensitive predictor for potential GPI lipid modification sites in fungal protein sequences and its application to genome-wide studies for Aspergillus nidulans, Candida albicans Neurospora crassa, Saccharomyces cerevisiae and Schizosaccharomyces pombeJournal of Molecular Biology, 2004
- Gapped BLAST and PSI-BLAST: a new generation of protein database search programsNucleic Acids Research, 1997
- Toward Defining the Course of Evolution: Minimum Change for a Specific Tree TopologySystematic Zoology, 1971