Ctf19p: A Novel Kinetochore Protein in Saccharomyces cerevisiae and a Potential Link between the Kinetochore and Mitotic Spindle
Open Access
- 5 April 1999
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 145 (1), 15-28
- https://doi.org/10.1083/jcb.145.1.15
Abstract
A genetic synthetic dosage lethality (SDL) screen using CTF13 encoding a known kinetochore protein as the overexpressed reference gene identified two chromosome transmission fidelity (ctf) mutants, YCTF58 and YCTF26. These mutant strains carry independent alleles of a novel gene, which we have designated CTF19. In light of its potential role in kinetochore function, we have cloned and characterized the CTF19 gene in detail. CTF19 encodes a nonessential 369–amino acid protein. ctf19 mutant strains display a severe chromosome missegregation phenotype, are hypersensitive to benomyl, and accumulate at G2/M in cycling cells. CTF19 genetically interacts with kinetochore structural mutants and mitotic checkpoint mutants. In addition, ctf19 mutants show a defect in the ability of centromeres on minichromosomes to bind microtubules in an in vitro assay. In vivo cross-linking and chromatin immunoprecipitation demonstrates that Ctf19p specifically interacts with CEN DNA. Furthermore, Ctf19-HAp localizes to the nuclear face of the spindle pole body and genetically interacts with a spindle-associated protein. We propose that Ctf19p is part of a macromolecular kinetochore complex, which may func- tion as a link between the kinetochore and the mitotic spindle.Keywords
This publication has 57 references indexed in Scilit:
- Analysis of the Saccharomyces Spindle Pole by Matrix-assisted Laser Desorption/Ionization (MALDI) Mass SpectrometryThe Journal of cell biology, 1998
- Life with 6000 GenesScience, 1996
- Aberrantly segregating centromeres activate the spindle assembly checkpoint in budding yeast.The Journal of cell biology, 1996
- Gene disruption with PCR products in Saccharomyces cerevisiaeGene, 1995
- Mad1p, a phosphoprotein component of the spindle assembly checkpoint in budding yeast.The Journal of cell biology, 1995
- Kinetochores, microtubules and the metaphase checkpointTrends in Cell Biology, 1995
- CEP3 encodes a centromere protein of Saccharomyces cerevisiae.The Journal of cell biology, 1995
- NDC10: a gene involved in chromosome segregation in Saccharomyces cerevisiae.The Journal of cell biology, 1993
- Feedback control of mitosis in budding yeastCell, 1991
- S. cerevisiae genes required for cell cycle arrest in response to loss of microtubule functionCell, 1991