TACE release of TNF-α mediates mechanotransduction-induced activation of p38 MAPK and myogenesis
Open Access
- 15 February 2007
- journal article
- Published by The Company of Biologists in Journal of Cell Science
- Vol. 120 (4), 692-701
- https://doi.org/10.1242/jcs.03372
Abstract
Skeletal muscle responds to mechanical stimulation by activating p38 MAPK, a key signal for myogenesis. However, the mechanotransduction mechanism that activates p38 is unknown. Here we show that mechanical stimulation of myoblasts activates p38 and myogenesis through stimulating TNF-α release by TNF-α converting enzyme (TACE). In C2C12 or mouse primary myoblasts cultured in growth medium, static stretch activated p38 along with ERK1/2, JNK and AKT. Disrupting TNF-α signaling by TNF-α-neutralizing antibody or knocking out TNF-α receptors blocked stretch activation of p38, but not ERK1/2, JNK or AKT. Stretch also activated differentiation markers MEF2C, myogenin, p21 and myosin heavy chain in a TNF-α- and p38-dependent manner. Stretch stimulated the cleavage activity of TACE. Conversely, TACE inhibitor TAPI or TACE siRNA abolished stretch activation of p38. In addition, conditioned medium from stretched myoblast cultures activated p38 in unstretched myoblasts, which required TACE activity in the donor myoblasts, and TNF-α receptors in the recipient myoblasts. These results indicate that posttranscriptional activation of TACE mediates the mechanotransduction that activates p38-dependent myogenesis via the release of TNF-α.Keywords
This publication has 87 references indexed in Scilit:
- TNF-α regulates myogenesis and muscle regeneration by activating p38 MAPKAmerican Journal of Physiology-Cell Physiology, 2007
- Role of TNF-α signaling in regeneration of cardiotoxin-injured muscleAmerican Journal of Physiology-Cell Physiology, 2005
- MyoD Targets Chromatin Remodeling Complexes to the Myogenin Locus Prior to Forming a Stable DNA-Bound ComplexMolecular and Cellular Biology, 2005
- ADAM12 and α9β1Integrin Are Instrumental in Human Myogenic Cell DifferentiationMolecular Biology of the Cell, 2005
- A MyoD-generated feed-forward circuit temporally patterns gene expression during skeletal muscle differentiationGenes & Development, 2004
- Differential regulation of the phosphoinositide 3-kinase and MAP kinase pathways by hepatocyte growth factor vs. insulin-like growth factor-I in myogenic cellsExperimental Cell Research, 2004
- Characterization of Growth Factor-induced Serine Phosphorylation of Tumor Necrosis Factor-α Converting Enzyme and of an Alternatively Translated PolypeptidePublished by Elsevier BV ,2003
- Tumor Necrosis Factor-α (TNF-α) Stimulates Chemotactic Response in Mouse Myogenic CellsCell Transplantation, 2003
- Transforming Growth Factor-β Inhibition of Insulin-like Growth Factor-binding Protein-5 Synthesis in Skeletal Muscle Cells Involves a c-Jun N-terminal Kinase-dependent PathwayPublished by Elsevier BV ,2001
- Mechanical Stretch Induces Activation of Skeletal Muscle Satellite Cells in VitroExperimental Cell Research, 2001