Surface Functionalization of Titanium with Chitosan/Gelatin via Electrophoretic Deposition: Characterization and Cell Behavior

Abstract

The electrophoretic deposition (EPD) is a versatile and cost-effective technique for fabricating advanced coatings. In this study, chitosan/gelatin (CS/G) coatings were prepared on titanium substrates via EPD. The prepared coatings were characterized using fluorescence microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and shear bond strength testing. It was found that CS/G coatings had a similar macroporous structure. The gelatin content in the CS/G coatings gradually increased with the increase of the gelatin in the blend solutions. The shear bond strength of the CS/G coatings also increased with the increasing gelatin content. In vitro biological tests demonstrated that human MG63 osteoblast-like cells achieved better affinity on the coatings with higher gelatin content. Therefore, it was concluded that EPD was an effective and efficient technique to prepare CS/G coatings on the titanium surface and that CS/G coatings with higher gelatin content were promising candidates for further loading of functional agents.