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Data from A Panel of Four miRNAs Accurately Differentiates Malignant from Benign Indeterminate Thyroid Lesions on Fine Needle Aspiration
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Data from A Panel of Four miRNAs Accurately Differentiates Malignant from Benign Indeterminate Thyroid Lesions on Fine Needle Aspiration
Data from A Panel of Four miRNAs Accurately Differentiates Malignant from Benign Indeterminate Thyroid Lesions on Fine Needle Aspiration
XK
Xavier M. Keutgen
Xavier M. Keutgen
FF
Filippo Filicori
Filippo Filicori
MC
Michael J. Crowley
Michael J. Crowley
YW
Yongchun Wang
Yongchun Wang
TS
Theresa Scognamiglio
Theresa Scognamiglio
RH
Rana Hoda
Rana Hoda
DB
Daniel Buitrago
Daniel Buitrago
DC
David Cooper
David Cooper
MZ
Martha A. Zeiger
Martha A. Zeiger
RZ
Rasa Zarnegar
Rasa Zarnegar
OE
Olivier Elemento
Olivier Elemento
TF
Thomas J. Fahey
Thomas J. Fahey
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31 March 2023
other
Published by
American Association for Cancer Research (AACR)
https://doi.org/10.1158/1078-0432.c.6520379.v1
Abstract
Purpose: Indeterminate thyroid lesions on fine needle aspiration (FNA) harbor malignancy in about 25% of cases. Hemi- or total thyroidectomy has, therefore, been routinely advocated for definitive diagnosis. In this study, we analyzed miRNA expression in indeterminate FNA samples and determined its prognostic effects on final pathologic diagnosis.Experimental Design: A predictive model was derived using 29 ex vivo indeterminate thyroid lesions on FNA to differentiate malignant from benign tumors at a tertiary referral center and validated on an independent set of 72 prospectively collected in vivo FNA samples. Expression levels of miR-222, miR-328, miR-197, miR-21, miR-181a, and miR-146b were determined using reverse transcriptase PCR. A statistical model was developed using the support vector machine (SVM) approach.Results: A SVM model with four miRNAs (miR-222, miR-328, miR-197, and miR-21) was initially estimated to have 86% predictive accuracy using cross-validation. When applied to the 72 independent in vivo validation samples, performance was actually better than predicted with a sensitivity of 100% and specificity of 86%, for a predictive accuracy of 90% in differentiating malignant from benign indeterminate lesions. When Hurthle cell lesions were excluded, overall accuracy improved to 97% with 100% sensitivity and 95% specificity.Conclusions: This study shows that that the expression of miR-222, miR-328, miR-197, and miR-21 combined in a predictive model is accurate at differentiating malignant from benign indeterminate thyroid lesions on FNA. These findings suggest that FNA miRNA analysis could be a useful adjunct in the management algorithm of patients with thyroid nodules. Clin Cancer Res; 18(7); 2032–8. ©2012 AACR.
Keywords
FNA
LESIONS
ACCURATELY DIFFERENTIATES MALIGNANT
ACCURATE AT DIFFERENTIATING
BENIGN INDETERMINATE THYROID
MIRNAS
PREDICTIVE MODEL
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