Soluble browning reaction pigments of aged Burley tobacco. I. The nondialyzable fraction
- 1 January 1960
- journal article
- research article
- Published by Elsevier BV in Archives of Biochemistry and Biophysics
- Vol. 86 (1), 94-101
- https://doi.org/10.1016/0003-9861(60)90375-1
Abstract
A dark-brown pigment, identified as an iron-protein-chlorogenic acid-rutin complex, was isolated by precipitation with acetic acid from the soluble nondialyzable fraction of aged Burley tobacco in an amount corresponding to 0.32% of dry leaf weight. Identification was made by paper chromatography of the products of hydrolysis and potassium hydroxide fusion. The acetic acid-insoluble pigment, an acetic acid-soluble brown pigment of similar qualitative composition, and a polysaccharide comprised the total acetone-precipitated nondialyzable fraction. Identification of the natural pigment confirms previous in vitro conclusions regarding the participation of chlorogenic acid and rutin in the browning of air-cured tobacco.Keywords
This publication has 6 references indexed in Scilit:
- The quantitative determination of amino acids by paper chromatography A solvent to replace phenolBiochimica et Biophysica Acta, 1957
- Fruit Juice Constituents, Chromatographic Comparison of Nonvolatile Acids of Fresh and Stored Apple Juice ConcentrateJournal of Agricultural and Food Chemistry, 1955
- Studies on the Browning and Blackening of Plant TissuesPhysiologia Plantarum, 1955
- A Fluorescence Test for Serotonin and Other TryptaminesNature, 1953
- Amino Acid Composition of Crystalline Inorganic Pyrophosphatase Isolated from Bakers YeastJournal of the American Chemical Society, 1952
- Paper Chromatography of Anthraquinone PigmentsJournal of the American Chemical Society, 1950