Selective Proliferation of Lactic Acid Bacteria and Accumulation of Lactic Acid during Open Fermentation of Kitchen Refuse with Intermittent pH Adjustment.
- 1 January 2000
- journal article
- Published by Japanese Society for Food Science and Technology in Food Science and Technology Research
- Vol. 6 (2), 140-145
- https://doi.org/10.3136/fstr.6.140
Abstract
When minced and autoclaved model kitchen refuse was inoculated with a small amount of non-autoclaved model kitchen refuse as seed culture, incubated at 37°C for 3-5 days and intermittently pH neutralized, 27-45 g/l of lactic acid was accumulated with a small amount of acetic acid and ethanol. The highest accumulation and highest productivity levels of lactic acid were observed at an initial and adjusted pH of 7.0 and a 6 h interval of pH adjustment. After several hours of lag, the lactic acid bacteria became the dominant cell type during the incubation, while the number of coliform bacteria and clostridia decreased. Such selective and stable accumulation of lactic acid was achieved in dozens of different experiments with various refuse preparations. In contrast, with continuous pH adjustment, Iactic acid once accumulated was labile and a small amount of butyric acid was produced, increasing the number of clostridia. The dominant bacteria isolated from the fermentation with intermittent pH adjustment were identified as Lactobacillus plantarnm and L. brevis.Keywords
This publication has 13 references indexed in Scilit:
- GenBankNucleic Acids Research, 1998
- Recycling of bakery wastes using an amylolytic lactic acid bacteriumBioresource Technology, 1997
- The RDP (Ribosomal Database Project)Nucleic Acids Research, 1997
- Production of lactic acid from wastepaper as a cellulosic feedstockJournal of Industrial Microbiology & Biotechnology, 1997
- Evaluation of nitrogen supplements for bioconversion of municipal solid waste to lactic acidApplied Biochemistry and Biotechnology, 1996
- Microbiological Production of Lactic AcidAdvances in Applied Microbiology, 1996
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994
- Sugar-cane pressmud as a novel and inexpensive substrate for production of lactic acid in a solid-state fermentation systemApplied Microbiology and Biotechnology, 1994
- Lactic Acid Fermentation of Broiler Processing Waste: Physical Properties and Chemical AnalysesPoultry Science, 1992
- Effect of lactobacilli inoculation on cassava (Manihot esculenta) silage: Fermentation pattern and kinetic analysisJournal of the Science of Food and Agriculture, 1990