Membrane Integration of Poliovirus 2B Viroporin
- 1 November 2011
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 85 (21), 11315-11324
- https://doi.org/10.1128/jvi.05421-11
Abstract
Virus infections can result in a variety of cellular injuries, and these often involve the permeabilization of host membranes by viral proteins of the viroporin family. Prototypical viroporin 2B is responsible for the alterations in host cell membrane permeability that take place in enterovirus-infected cells. 2B protein can be localized at the endoplasmic reticulum (ER) and the Golgi complex, inducing membrane remodeling and the blockade of glycoprotein trafficking. These findings suggest that 2B has the potential to integrate into the ER membrane, but specific information regarding its biogenesis and mechanism of membrane insertion is lacking. Here, we report experimental results of in vitro translation-glycosylation compatible with the translocon-mediated insertion of the 2B product into the ER membrane as a double-spanning integral membrane protein with an N-/C-terminal cytoplasmic orientation. A similar topology was found when 2B was synthesized in cultured cells. In addition, the in vitro translation of several truncated versions of the 2B protein suggests that the two hydrophobic regions cooperate to insert into the ER-derived microsomal membranes.This publication has 69 references indexed in Scilit:
- N‐glycosylation efficiency is determined by the distance to the C‐terminus and the amino acid preceding an Asn‐Ser‐Thr sequonProtein Science, 2010
- Membrane Insertion and Biogenesis of the Turnip Crinkle Virus p9 Movement ProteinJournal of Virology, 2010
- The 3-dimensional structure of a hepatitis C virus p7 ion channel by electron microscopyProceedings of the National Academy of Sciences of the United States of America, 2009
- Plant Virus Cell-to-Cell Movement Is Not Dependent on the Transmembrane Disposition of Its Movement ProteinJournal of Virology, 2009
- Transmembrane protein topology prediction using support vector machinesBMC Bioinformatics, 2009
- The Surfactant Peptide KL4 Sequence Is Inserted with a Transmembrane Orientation into the Endoplasmic Reticulum MembraneBiophysical Journal, 2008
- Functional Analysis of Picornavirus 2B Proteins: Effects on Calcium Homeostasis and Intracellular Protein TraffickingJournal of Virology, 2008
- Predicting transmembrane protein topology with a hidden markov model: application to complete genomesJournal of Molecular Biology, 2001
- Turns in transmembrane helices: determination of the minimal length of a “helical hairpin” and derivation of a fine-grained turn propensity scaleJournal of Molecular Biology, 1999
- A turn propensity scale for transmembrane helicesJournal of Molecular Biology, 1999