Depleted level of heparan sulfate proteoglycan in the extracellular matrix of endothelial cell cultures exposed to endotoxin
- 1 April 1994
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 159 (1), 121-130
- https://doi.org/10.1002/jcp.1041590116
Abstract
Exposure of cultured endothelial cells to endotoxin causes an increase in the amount of cellular heparan sulfate proteoglycan and a depletion of this molecule in the extracellular matrix. Concomitant with the decrease in the extracellular matrix is the appearance of a fraction of proteoglycan bearing altered carbohydrate moieties in the culture medium, β‐mereaptoethanol, mannitol, and dimethyl sulfoxide bring back to normal the structural properties of the proteoglycan in the medium and restore the matrix content in proteoglycan to a level comparable to that of control cells but do not affect the increase in the amount of proteoglycan on the cell. This “uncoupling” suggests that two independent chains of events underlie the synthetic and structural changes of the proteoglycan triggered by endotoxin in the endothelial cell.Keywords
This publication has 27 references indexed in Scilit:
- Heparan sulphate in the binding and activation of basic fibroblast growth factorGlycobiology, 1992
- Enhanced inhibition of tissue factor by the extended form of an endothelial cell glycoprotein (an extrinsic pathway inhibitor)Journal of Cellular Physiology, 1991
- Release of basic fibroblast growth factor-heparan sulfate complexes from endothelial cells by plasminogen activator-mediated proteolytic activity.The Journal of cell biology, 1990
- Matrix-associated heparan sulfate proteoglycan: core protein-specific monoclonal antibodies decorate the pericellular matrix of connective tissue cells and the stromal side of basement membranes.The Journal of cell biology, 1989
- Nitric oxide: A cytotoxic activated macrophage effector moleculeBiochemical and Biophysical Research Communications, 1988
- Cell surface proteoglycan binds mouse mammary epithelial cells to fibronectin and behaves as a receptor for interstitial matrixThe Journal of cell biology, 1988
- N-glycansulfated fibronectin: One of the several sulfated glycoproteins synthesized by endothelial cells in cultureBiochemical and Biophysical Research Communications, 1987
- Localization of binding sites for laminin, heparan sulfate proteoglycan and fibronectin on basement membrane (type IV) collagenJournal of Molecular Biology, 1986
- Cultured human endothelial cells generate tissue factor in response to endotoxin.JCI Insight, 1983
- Anti-clotting activity of endothelial cell cultures and heparan sulfate proteoglycansBiochemical and Biophysical Research Communications, 1982