Quantitative visualization of DNA G-quadruplex structures in human cells
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- 20 January 2013
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Chemistry
- Vol. 5 (3), 182-186
- https://doi.org/10.1038/nchem.1548
Abstract
Four-stranded G-quadruplex nucleic acid structures are of great interest as their high thermodynamic stability under near-physiological conditions suggests that they could form in cells. Here we report the generation and application of an engineered, structure-specific antibody employed to quantitatively visualize DNA G-quadruplex structures in human cells. We show explicitly that G-quadruplex formation in DNA is modulated during cell-cycle progression and that endogenous G-quadruplex DNA structures can be stabilized by a small-molecule ligand. Together these findings provide substantive evidence for the formation of G-quadruplex structures in the genome of mammalian cells and corroborate the application of stabilizing ligands in a cellular context to target G-quadruplexes and intervene with their function.Keywords
This publication has 25 references indexed in Scilit:
- Small-molecule–induced DNA damage identifies alternative DNA structures in human genesNature Chemical Biology, 2012
- A single-molecule platform for investigation of interactions between G-quadruplexes and small-molecule ligandsNature Chemistry, 2011
- DNA Replication through G-Quadruplex Motifs Is Promoted by the Saccharomyces cerevisiae Pif1 DNA HelicaseCell, 2011
- Epigenetic Instability due to Defective Replication of Structured DNAMolecular Cell, 2010
- Small-molecule-mediated G-quadruplex isolation from human cellsNature Chemistry, 2010
- An Alternative DNA Structure Is Necessary for Pilin Antigenic Variation in Neisseria gonorrhoeaeScience, 2009
- A Novel Small Molecule That Alters Shelterin Integrity and Triggers a DNA-Damage Response at TelomeresJournal of the American Chemical Society, 2008
- Conserved elements with potential to form polymorphic G-quadruplex structures in the first intron of human genesNucleic Acids Research, 2008
- Gene function correlates with potential for G4 DNA formation in the human genomeNucleic Acids Research, 2006
- Formation of parallel four-stranded complexes by guanine-rich motifs in DNA and its implications for meiosisNature, 1988