Amplifications of DNA primase 1 (PRIM1) in human osteosarcoma
- 1 September 1999
- journal article
- research article
- Published by Wiley in Genes, Chromosomes and Cancer
Abstract
We studied the involvement of PRIM1 in osteosarcoma by differential display, Northern and Southern hybridization, as well as fluorescence in situ hybridization (FISH) on interphase nuclei. In total, 22 pediatric oncology specimens were tested. PRIM1 was found to be amplified in 41% of the samples. PRIM1 is coamplified with the core 12q13 amplicon genes CDK4, SAS, and OS9, and was physically mapped very close to them. PRIM1 is therefore a new candidate for the role of a major target gene of 12q13 amplifications in human cancers. Genes Chromosomes Cancer 26:62–69, 1999.Keywords
This publication has 29 references indexed in Scilit:
- Mapping of the Human DNA Primase 1 (PRIM1) to Chromosome 12q13Genomics, 1997
- HMGIC, the gene for an architectural transcription factor, is amplified and rearranged in a subset of human sarcomasOncogene, 1997
- Transcript Mapping in a 46-kb Sequenced Region at the Core of 12q13.3 Amplification in Human CancersGenomics, 1997
- COMPARATIVE GENOMIC HYBRIDIZATION (CGH) ANALYSIS OF NEUROBLASTOMAS—AN IMPORTANT METHODOLOGICAL APPROACH IN PAEDIATRIC TUMOUR PATHOLOGYThe Journal of Pathology, 1997
- SAS is amplified predominantly in surface osteosarcomaJournal of Orthopaedic Research, 1996
- Complete sequence analysis of a gene (OS-9) ubiquitously expressed in human tissues and amplified in sarcomasMolecular Carcinogenesis, 1996
- MDM2 amplification in a primary alveolar rhabdomyosarcoma displaying a t(2;13)(q35;q14)Cytogenetic and Genome Research, 1996
- Identification of multiple chromosome 12 abnormalities in human testicular germ cell tumors by two‐color fluorescence in situ hybridization (FISH)Genes, Chromosomes and Cancer, 1995
- MDM2 and CDK4 gene amplification in Ewing's sarcomaThe Journal of Pathology, 1995
- The protooncogene CHOP/GADD153, involved in growth arrest and DNA damage response, is amplified in a subset of human sarcomasCancer Genetics and Cytogenetics, 1994