OLIGO 7 Primer Analysis Software
- 1 January 2007
- book chapter
- review article
- Published by Springer Science and Business Media LLC
- Vol. 402, 35-59
- https://doi.org/10.1007/978-1-59745-528-2_2
Abstract
OLIGO performs a range of functions for researches in PCR and related technologies such as PCR and sequencing primer selection, hybridization probe design, inverse and real-time PCR, analysis of false priming using a unique priming efficiency (PE) algorithm, design of consensus and multiplex, nested primers and degenerate primers, reverse translation, and restriction enzyme analysis and prediction; based on a protein sequence, oligonucleotide database allows fully automatic multiplexing, primer secondary structure analysis, and more. OLIGO allows for sequence file batch processing that is essential for automation. This chapter describes the major functions of OLIGO version 7 software.Keywords
This publication has 11 references indexed in Scilit:
- Functional siRNAs and miRNAs Exhibit Strand BiasCell, 2003
- Thermodynamic parameters for DNA sequences with dangling endsNucleic Acids Research, 2000
- Nearest-Neighbor Thermodynamics and NMR of DNA Sequences with Internal A·A, C·C, G·G, and T·T MismatchesBiochemistry, 1999
- Thermodynamic Parameters for an Expanded Nearest-Neighbor Model for Formation of RNA Duplexes with Watson−Crick Base PairsBiochemistry, 1998
- Thermodynamics of internal C.T mismatches in DNANucleic Acids Research, 1998
- Nearest-Neighbor Thermodynamics of Internal A·C Mismatches in DNA: Sequence Dependence and pH EffectsBiochemistry, 1998
- A unified view of polymer, dumbbell, and oligonucleotide DNA nearest-neighbor thermodynamicsProceedings of the National Academy of Sciences of the United States of America, 1998
- Nearest Neighbor Thermodynamic Parameters for Internal G·A Mismatches in DNABiochemistry, 1998
- Thermodynamics and NMR of Internal G·T Mismatches in DNABiochemistry, 1997
- Rapid and Sensitive Protein Similarity SearchesScience, 1985