Purification and properties of suppressor seryl‐tRNA:ATP phosphotransferase from bovine liver
- 24 April 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 169 (2), 319-322
- https://doi.org/10.1016/0014-5793(84)80342-7
Abstract
Seryl-tRNASer CmCA:ATP phosphotransferase was purified 1200-fold from bovine liver by ultracentrifugation at 150 000 × g, chromatography on DEAE—cellulose, fractional precipitation with ammonium sulfate, chromatography on hydroxyapatite, gel filtration on Sephacryl S-300 and affinity chromatography on Blue Sepharose. Molecular mass was estimated as 135–145 kDa. The K m values for ATP and ser-tRNASer CmCA were 2 mM and 21 nM, respectively. This enzyme did not react with ser-tRNASer IGA, tyr-tRNA or thr-tRNA.Keywords
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