Natural proteins: Sources, isolation, characterization and applications
Open Access
- 1 January 2013
- journal article
- review article
- Published by EManuscript Technologies in Pharmacognosy Reviews
- Vol. 7 (14), 107-116
- https://doi.org/10.4103/0973-7847.120508
Abstract
Worldwide, plant protein contributes substantially as a food resource because it contains essential amino acids for meeting human physiological requirements. However, many versatile plant proteins are used as medicinal agents as they are produced by using molecular tools of biotechnology. Proteins can be obtained from plants, animals and microorganism cells. The abundant economical proteins can be obtained from plant seeds. These natural proteins are obtained by isolation procedures depending on the physicochemical properties of proteins. Isolation and purification of single protein from cells containing mixtures of unrelated proteins is achievable due to the physical and chemical attributes of proteins. The following characteristics are unique to each protein: Amino acid composition, sequence, subunit structures, size, shape, net charge, isoelectric point, solubility, heat stability and hydrophobicity. Based on these properties, various methods of isolation exist, like salting out and isoionic precipitation. Purification of proteins is quiet challenging and, therefore, several approaches like sodium dodecyl sulfate gel electrophoresis and chromatography are available. Characterization of proteins can be performed by mass spectrometry/liquid chromatography-mass spectrometry (LC-MS). The amino acid sequence of a protein can be detected by using tandem mass spectrometry. In this article, a review has been made on the sources, isolation, purification and characterization of natural proteins.Keywords
This publication has 34 references indexed in Scilit:
- Silk-based delivery systems of bioactive moleculesAdvanced Drug Delivery Reviews, 2010
- Collisions or Electrons? Protein Sequence Analysis in the 21st CenturyAnalytical Chemistry, 2009
- Silk-based biomaterialsBiomaterials, 2003
- Isolation of pisumin, a novel antifungal protein from legumes of the sugar snap pea Pisum sativum var. macrocarponComparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 2003
- The nutritional value of plant-based diets in relation to human amino acid and protein requirementsProceedings of the Nutrition Society, 1999
- Conformational effects in reversed-phase high-performance liquid chromatography of polypeptides II. The role of insulin A and B chains in the chromatographic behaviour of insulinJournal of Chromatography A, 1995
- Whey protein concentrates and isolates: Processing and functional propertiesCritical Reviews in Food Science and Nutrition, 1993
- Milk proteins: Physicochemical and functional propertiesC R C Critical Reviews in Food Science and Nutrition, 1984
- A simple method for displaying the hydropathic character of a proteinJournal of Molecular Biology, 1982
- Thermostability and Aliphatic Index of Globular ProteinsThe Journal of Biochemistry, 1980