Survival of Cold-Stressed Campylobacter jejuni on Ground Chicken and Chicken Skin during Frozen Storage
Open Access
- 1 December 2004
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 70 (12), 7103-7109
- https://doi.org/10.1128/aem.70.12.7103-7109.2004
Abstract
Campylobacter jejuni is prevalent in poultry, but the effect of combined refrigerated and frozen storage on its survival, conditions relevant to poultry processing and storage, has not been evaluated. Therefore, the effects of refrigeration at 4°C, freezing at −20°C, and a combination of refrigeration and freezing on the survival of C. jejuni in ground chicken and on chicken skin were examined. Samples were enumerated using tryptic soy agar containing sheep's blood and modified cefoperazone charcoal deoxycholate agar. Refrigerated storage alone for 3 to 7 days produced a reduction in cell counts of 0.34 to 0.81 log 10 CFU/g in ground chicken and a reduction in cell counts of 0.31 to 0.63 log 10 CFU/g on chicken skin. Declines were comparable for each sample type using either plating medium. Frozen storage, alone and with prerefrigeration, produced a reduction in cell counts of 0.56 to 1.57 log 10 CFU/g in ground chicken and a reduction in cell counts of 1.38 to 3.39 log 10 CFU/g on chicken skin over a 2-week period. The recovery of C. jejuni following freezing was similar on both plating media. The survival following frozen storage was greater in ground chicken than on chicken skin with or without prerefrigeration. Cell counts after freezing were lower on chicken skin samples that had been prerefrigerated for 7 days than in those that had been prerefrigerated for 0, 1, or 3 days. This was not observed for ground chicken samples, possibly due to their composition. C. jejuni survived storage at 4 and −20°C with either sample type. This study indicates that, individually or in combination, refrigeration and freezing are not a substitute for safe handling and proper cooking of poultry.Keywords
This publication has 13 references indexed in Scilit:
- Direct Microscopic Observation and Viability Determination of Campylobacter jejuni on Chicken SkinJournal of Food Protection, 2003
- Effect of Temperature on Viability of Campylobacter jejuni and Campylobacter coli on Raw Chicken or Pork SkinJournal of Food Protection, 2003
- Survival of Campylobacter jejuni on beef trimmings during freezing and frozen storageLetters in Applied Microbiology, 2002
- Food-Related Illness and Death in the United StatesEmerging Infectious Diseases, 1999
- Aerobic growth and survival of Campylobacter jejuni in food and stream water.Letters in Applied Microbiology, 1998
- Survival and Growth of Campylobacter jejuni after Artificial Inoculation onto Chicken Skin as a Function of Temperature and Packaging ConditionsJournal of Food Protection, 1998
- Resistance ofListeria monocytogenes to freezing in foodsFood Microbiology, 1991
- Efficacy of some methods and media for detecting and enumeratingCampylobacter jejuniin frozen chicken meatJournal of Applied Bacteriology, 1987
- Injury and recovery in freeze- or heat-damaged Campylobacter jejuniLetters in Applied Microbiology, 1986
- Survival and growth of Campylobacter fetus subsp. jejuni on meat and in cooked foodsApplied and Environmental Microbiology, 1982