Impaired Somatodendritic Responses to Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) of Supraoptic Neurones in PACAP type I -Receptor Deficient Mice
- 4 August 2003
- journal article
- Published by Wiley in Journal of Neuroendocrinology
- Vol. 15 (9), 871-881
- https://doi.org/10.1046/j.1365-2826.2003.01075.x
Abstract
The role of pituitary adenylate cyclase-activating polypeptide (PACAP) type I receptor (PAC1 receptor) in regulating hypothalamic supraoptic neurones was investigated using PAC1 receptor-deficient male mice (PAC1-/-). The effects of PACAP on [Ca2+]i were investigated in freshly dissociated supraoptic neurones and on the somatodendritic release of vasopressin and oxytocin, examined on intact supraoptic nuclei. In supraoptic neurones from wild-type mice (PAC1+/+), 100 nm PACAP induced an increase in [Ca2+]i and release of vasopressin and oxytocin, whereas in heterozygous (PAC1+/-) and null-mutant mice (PAC1-/-), PACAP was much less effective. PACAP had no effect on these two parameters when applied to isolated neurohypophysial nerve terminals of PAC1+/+ and PAC1-/- mice, and rats. In conclusion, the PAC1 receptor is solely responsible for the PACAP-induced [Ca2+]i signalling and secretion of vasopressin and oxytocin in the somatodendritic region of supraoptic neurones.Keywords
This publication has 38 references indexed in Scilit:
- Pituitary Adenylate Cyclase-Activating Polypeptide and Its Type I Receptors in the Rat Hypothalamus: Neuroendocrine InteractionsaAnnals of the New York Academy of Sciences, 2006
- Activation of multiple intracellular transduction signals by vasopressin in vasopressin‐sensitive neurones of the rat supraoptic nucleusThe Journal of Physiology, 1998
- Molecular Cloning of a Novel Variant of the Pituitary Adenylate Cyclase-activating Polypeptide (PACAP) Receptor That Stimulates Calcium Influx by Activation of L-type Calcium ChannelsPublished by Elsevier BV ,1996
- The expression of pituitary adenylate cyclase-activating polypeptide (PACAP) mRNA in rat brain: possible role of endogenous PACAP in vasopressin releaseNeuroscience Letters, 1995
- Localization of Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) in the Hypothalamus-Pituitary System in Rats: Light and Electron Microscopic Immunocytochemical StudiesJournal of Neuroendocrinology, 1994
- Multiple receptors for PACAP and VIPTrends in Pharmacological Sciences, 1994
- Pituitary Adenylate Cyclase-Activating Polypeptide Stimulates Arginine Vasopressin Release in Conscious RatsNeuroendocrinology, 1993
- Isolation of a neuropeptide corresponding to the N-terminal 27 residues of the pituitary adenylate cyclase activating polypeptide with 38 residues (PACAP38)Biochemical and Biophysical Research Communications, 1990
- Isolation of a novel 38 residue-hypothalamic polypeptide which stimulates adenylate cyclase in pituitary cellsBiochemical and Biophysical Research Communications, 1989