Plzf is required in adult male germ cells for stem cell self-renewal
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- 23 May 2004
- journal article
- letter
- Published by Springer Science and Business Media LLC in Nature Genetics
- Vol. 36 (6), 647-652
- https://doi.org/10.1038/ng1366
Abstract
Adult germline stem cells are capable of self-renewal, tissue regeneration and production of large numbers of differentiated progeny. We show here that the classical mouse mutant luxoid affects adult germline stem cell self-renewal. Young homozygous luxoid mutant mice produce limited numbers of normal spermatozoa and then progressively lose their germ line after birth. Transplantation studies showed that germ cells from mutant mice did not colonize recipient testes, suggesting that the defect is intrinsic to the stem cells. We determined that the luxoid mutant contains a nonsense mutation in the gene encoding Plzf, a transcriptional repressor that regulates the epigenetic state of undifferentiated cells, and showed that Plzf is coexpressed with Oct4 in undifferentiated spermatogonia. This is the first gene shown to be required in germ cells for stem cell self-renewal in mammals.Keywords
This publication has 28 references indexed in Scilit:
- Increment of Murine Spermatogonial Cell Number by Gonadotropin-Releasing Hormone Analogue Is Independent of Stem Cell Factor c-kit Signal1Biology of Reproduction, 2003
- Bmi-1 is required for maintenance of adult self-renewing haematopoietic stem cellsNature, 2003
- Bmi-1 determines the proliferative capacity of normal and leukaemic stem cellsNature, 2003
- A comprehensive genetic map of the mouse genomeNature, 1996
- The POZ domain: a conserved protein-protein interaction motif.Genes & Development, 1994
- A genetic map of the mouse with 4,006 simple sequence length polymorphismsNature Genetics, 1994
- A quantitative study of spermatogonial multiplication and stem cell renewal in the C3H/101 F1 hybrid mouseMutation Research, 1993
- Genome Maps IV 1993. Wall ChartScience, 1993
- Protein constituents of the mouse spermatozoonDevelopmental Biology, 1980
- The skeletal effects of the luxoid gene in the mouse, including its interactions withthe luxate gene.Journal of Morphology, 1958