Measurement of Adhesive Forces between Individual Staphylococcus aureus MSCRAMMs and Protein-Coated Surfaces by Use of Optical Tweezers
Open Access
- 15 March 2003
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 185 (6), 2031-2035
- https://doi.org/10.1128/jb.185.6.2031-2035.2003
Abstract
The force required to rupture bonds between individual Staphylococcus aureus MSCRAMMs and surfaces coated with extracellular matrix molecules has been quantified by using optical tweezers. The observed binding forces between fibrinogen or fibronectin and S. aureus MSCRAMMs occurred as an approximate integer multiple of 20 or 25 pN, respectively.Keywords
This publication has 24 references indexed in Scilit:
- Heterogeneity in Bacterial Surface Polysaccharides, Probed on a Single-Molecule BasisBiomacromolecules, 2002
- Ultralarge multimers of von Willebrand factor form spontaneous high-strength bonds with the platelet glycoprotein Ib-IX complex: studies using optical tweezersBlood, 2002
- Stress Response in Caenorhabditis elegans Caused by Optical Tweezers: Wavelength, Power, and Time DependenceBiophysical Journal, 2002
- Measuring the forces involved in polyvalent adhesion of uropathogenic Escherichia coli to mannose-presenting surfacesProceedings of the National Academy of Sciences, 2000
- Unbinding process of adsorbed proteins under external stress studied by atomic force microscopy spectroscopyProceedings of the National Academy of Sciences of the United States of America, 2000
- Characterization of Photodamage to Escherichia coli in Optical TrapsBiophysical Journal, 1999
- Adhesion Forces between E. coli Bacteria and Biomaterial SurfacesLangmuir, 1999
- Wavelength dependence of cell cloning efficiency after optical trappingBiophysical Journal, 1996
- Two different genes encode fibronectin binding proteins in Staphylococcus aureusEuropean Journal of Biochemistry, 1991
- Biomaterial-Centered Infection: Microbial Adhesion Versus Tissue IntegrationScience, 1987