Anaerobic oxidation of methane at different temperature regimes in Guaymas Basin hydrothermal sediments
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Open Access
- 17 November 2011
- journal article
- research article
- Published by Oxford University Press (OUP) in The ISME Journal
- Vol. 6 (5), 1018-1031
- https://doi.org/10.1038/ismej.2011.164
Abstract
Anaerobic oxidation of methane (AOM) was investigated in hydrothermal sediments of Guaymas Basin based on δ13C signatures of CH4, dissolved inorganic carbon and porewater concentration profiles of CH4 and sulfate. Cool, warm and hot in-situ temperature regimes (15–20 °C, 30–35 °C and 70–95 °C) were selected from hydrothermal locations in Guaymas Basin to compare AOM geochemistry and 16S ribosomal RNA (rRNA), mcrA and dsrAB genes of the microbial communities. 16S rRNA gene clone libraries from the cool and hot AOM cores yielded similar archaeal types such as Miscellaneous Crenarchaeotal Group, Thermoproteales and anaerobic methane-oxidizing archaea (ANME)-1; some of the ANME-1 archaea formed a separate 16S rRNA lineage that at present seems to be limited to Guaymas Basin. Congruent results were obtained by mcrA gene analysis. The warm AOM core, chemically distinct by lower porewater sulfide concentrations, hosted a different archaeal community dominated by the two deep subsurface archaeal lineages Marine Benthic Group D and Marine Benthic Group B, and by members of the Methanosarcinales including ANME-2 archaea. This distinct composition of the methane-cycling archaeal community in the warm AOM core was confirmed by mcrA gene analysis. Functional genes of sulfate-reducing bacteria and archaea, dsrAB, showed more overlap between all cores, regardless of the core temperature. 16S rRNA gene clone libraries with Euryarchaeota-specific primers detected members of the Archaeoglobus clade in the cool and hot cores. A V6-tag high-throughput sequencing survey generally supported the clone library results while providing high-resolution detail on archaeal and bacterial community structure. These results indicate that AOM and the responsible archaeal communities persist over a wide temperature range.This publication has 47 references indexed in Scilit:
- Thermophilic anaerobic oxidation of methane by marine microbial consortiaThe ISME Journal, 2011
- Identification of the dominant sulfate‐reducing bacterial partner of anaerobic methanotrophs of the ANME‐2 cladeEnvironmental Microbiology, 2010
- Spatial Structure and Activity of Sedimentary Microbial Communities Underlying a Beggiatoa spp. Mat in a Gulf of Mexico Hydrocarbon SeepPLOS ONE, 2010
- Anaerobic Oxidation of Methane: Progress with an Unknown ProcessAnnual Review of Microbiology, 2009
- Methane oxidation at 55°C and pH 2 by a thermoacidophilic bacterium belonging to the Verrucomicrobia phylumProceedings of the National Academy of Sciences of the United States of America, 2008
- An Anaerobic Methane-Oxidizing Community of ANME-1b Archaea in Hypersaline Gulf of Mexico SedimentsApplied and Environmental Microbiology, 2006
- Microbial Diversity of Hydrothermal Sediments in the Guaymas Basin: Evidence for Anaerobic Methanotrophic CommunitiesApplied and Environmental Microbiology, 2002
- Stable isotope tracing of anaerobic methane oxidation in the gassy sediments of Eckernfoerde Bay, German Baltic SeaAmerican Journal of Science, 1999
- Generation of short chain acid anions in hydrothermally altered sediments of the Guaymas Basin, Gulf of CaliforniaApplied Geochemistry, 1990
- Geochemistry of interstitial gases in Quaternary sediments of the Gulf of CaliforniaChemical Geology, 1984