Influence of Uraemia on the Determination of Blood Glycohaemoglobin by HPLC, Electrophoresis and Affinity Chromatography in Diabetic and Non-Diabetic Patients
- 1 January 1994
- journal article
- research article
- Published by Walter de Gruyter GmbH in cclm
- Vol. 32 (5), 361-364
- https://doi.org/10.1515/cclm.1994.32.5.361
Abstract
Glycohaemoglobin in human blood can be determined by several methods. We investigated three methods used in daily practice, viz. affinity chromatography, HPLC and electrophoresis. We examined the influence of uraemia in diabetic and non-diabetic patients on the results obtained with HPLC and electrophoresis and compared these with the results obtained with affinity chromatography. Our current method (electrophoresis) proved to be very sensitive to uraemia giving falsely elevated levels of HbA1. HPLC was not disturbed by uraemia.Keywords
This publication has 3 references indexed in Scilit:
- Glycated Haemoglobin Measurement in Uraemic PatientsAnnals of Clinical Biochemistry: International Journal of Laboratory Medicine, 1991
- Comparison of Three Different Assay Procedures for the Determination of HbA1 with Special Attention to the Influence of Pre-HbA1c, Temperature and Haemoglobin ConcentrationAnnals of Clinical Biochemistry: International Journal of Laboratory Medicine, 1985
- Glycosylated or Carbamylated Hemoglobin in Uremia?The New England Journal of Medicine, 1982