Effect of Donor Age on Function of Isolated Human Islets
- 1 May 2006
- journal article
- Published by American Diabetes Association in Diabetes
- Vol. 55 (5), 1361-1368
- https://doi.org/10.2337/db05-1333
Abstract
This study intended to evaluate the impact of donor age on the function of isolated islets. Analysis of human islets from cadaveric donors (age 16–70 years) was performed using glucose-stimulated insulin release (GSIR) (n = 93), islet ATP content (n = 27), diabetic nude mouse bioassay (n = 72), and the insulin secretory function after single-donor clinical islet allotransplantation (n = 7). The GSIR index was significantly higher in younger donors (age ≤40 years) than in older donors and negatively correlated with the donor age (r = −0.535). Islet ATP was higher in younger donors (115.7 ± 17.7 vs. 75.7 ± 6.6 pmol/μg DNA). The diabetes reversal rate of mice with 2,000 IE was significantly higher in younger donors (96 vs. 68%). C-peptide increment to glucose during intravenous glucose tolerance test at days 90–120 after clinical transplantation showed negative correlation with donor age (r = −0.872) and positive correlation with the islet mass (r = 0.832). On the other hand, acute insulin response to arginine only showed correlation with the islet mass and not with donor age. These results show that insulin secretory response to glucose deteriorates with increasing age and that it may be related to changes in ATP generation in β-cells.Keywords
This publication has 53 references indexed in Scilit:
- Impact of two-layer pancreas preservation on islet isolation and transplantation1Transplantation, 2002
- Diminished Insulin Secretory Response to Glucose but Normal Insulin and Glucagon Secretory Responses to Arginine in a Family With Maternally Inherited Diabetes and Deafness Caused by Mitochondrial tRNALEU(UUR) Gene MutationDiabetes Care, 2001
- The natural history of insulin secretory dysfunction and insulin resistance in the pathogenesis of type 2 diabetes mellitusJCI Insight, 1999
- RENAL TRANSPLANTATION IN RECIPIENTS OVER THE AGE OF 60Transplantation, 1999
- Increased Disorderliness of Basal Insulin Release, Attenuated Insulin Secretory Burst Mass, and Reduced Ultradian Rhythmicity of Insulin Secretion in Older IndividualsJournal of Clinical Endocrinology & Metabolism, 1997
- Mitochondrial decay in agingBiochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 1995
- Effect of age and sex on rat endocrine pancreasDiabetes, 1987
- The nature of insulin secretory defect in aging ratsDiabetes, 1985
- Structure and function changes in the endocrine pancreas of aging rats with reference to the modulating effects of exercise and caloric restriction.JCI Insight, 1981
- Effect of age on glucose-stimulated insulin release by the beta-cell of the rat.JCI Insight, 1979