FVB.129P2-Pde6b+Tyrc-ch/Ant, a sighted variant of the FVB/N mouse strain suitable for behavioral analysis
- 1 August 2007
- journal article
- research article
- Published by Wiley in Genes, Brain and Behavior
- Vol. 6 (6), 552-557
- https://doi.org/10.1111/j.1601-183x.2006.00282.x
Abstract
Mice of the FVB/N strain are severely visual impaired as a result of tyrosinase gene defects, leading to a deficiency of the key enzyme for melanin synthesis in skin and eye and of cyclic guanosine monophosphate phosphodiesterase gene defects, which results in albinism (Tyr(c/c)) and retinal degeneration (Pde6b(rd1/rd1)), respectively. Nevertheless, FVB/N mice are commonly used for the generation of transgenic animals because of their large, strong pronuclei and high breeding performance. However, due to visual impairment of the FVB/N animals, the resulting transgenic animals cannot be used in tests that depend on vision, including tests of cognitive behavior. Therefore, we have bred a sighted version of the FVB/N strain by an outcross between FVB/N and 129P2/OlaHsd, followed by repeated backcrosses to FVB/N mice while selecting against albinism and homozygosity of the retinal degeneration mutation. After 11 generations of backcrossing, sighted animals were intercrossed to generate the congenic FVB.129P2-Pde6b(+) Tyr(c-ch)/Ant strain, which is pigmented (Tyr(c-ch)/(c-ch)) and devoid of the genetic predisposition to retinal degeneration. The accurate visual abilities of the FVB.129P2-Pde6b(+) Tyr(c-ch)/Ant mice, for which we propose the name FVBS/Ant, demonstrated a clear visual evoked potential in the presence of normal eye histology and improved performance in the Morris water maze test.Keywords
This publication has 30 references indexed in Scilit:
- Fragile X Mental Retardation Protein Shifts between Polyribosomes and Stress Granules after Neuronal Injury by Arsenite Stress orIn VivoHippocampal Electrode InsertionJournal of Neuroscience, 2006
- Visual detection, pattern discrimination and visual acuity in 14 strains of miceGenes, Brain and Behavior, 2005
- Effects of the rd1 Mutation and Host Strain on Hippocampal Learning in MiceBehavior Genetics, 2005
- Deficits in Trace Fear Memory and Long-Term Potentiation in a Mouse Model for Fragile X SyndromeJournal of Neuroscience, 2005
- Applications of the Morris water maze in the study of learning and memoryBrain Research Reviews, 2001
- Anxiety-related behaviors in the elevated zero-maze are affected by genetic factors and retinal degeneration.Behavioral Neuroscience, 2001
- Hippocampal mossy fibers and swimming navigation in mice: Correlations with size and left‐right asymmetriesHippocampus, 1994
- Development and degeneration of retina in rds mutant mice: immunoassay of the rod visual pigment rhodopsinBiochimica et Biophysica Acta (BBA) - General Subjects, 1990
- Place navigation impaired in rats with hippocampal lesionsNature, 1982
- Preliminary report of a simple animal behavior model for the anxiolytic effects of benzodiazepinesPharmacology Biochemistry and Behavior, 1980