Induction of an interferon response by RNAi vectors in mammalian cells
- 8 June 2003
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Genetics
- Vol. 34 (3), 263-264
- https://doi.org/10.1038/ng1173
Abstract
DNA vectors that express short hairpin RNAs (shRNAs) from RNA polymerase III (Pol III) promoters are a promising new tool to reduce gene expression in mammalian cells. shRNAs are processed to small interfering RNAs (siRNAs) of 21 nucleotides (nt) that guide the cleavage of the cognate mRNA by the RNA-induced silencing complex. Although siRNAs are thought to be too short to induce interferon expression, we report here that a substantial number of shRNA vectors can trigger an interferon response.Keywords
This publication has 6 references indexed in Scilit:
- Role for the Mortality Factors MORF4, MRGX, and MRG15 in Transcriptional Repression via Associations with Pf1, mSin3A, and Transducin-Like Enhancer of SplitMolecular and Cellular Biology, 2002
- A System for Stable Expression of Short Interfering RNAs in Mammalian CellsScience, 2002
- Exploiting tumor-specific defects in the interferon pathway with a previously unknown oncolytic virusNature Medicine, 2000
- Identification of a Gene That Reverses the Immortal Phenotype of a Subset of Cells and Is a Member of a Novel Family of Transcription Factor-Like GenesMolecular and Cellular Biology, 1999
- In Vivo Gene Delivery and Stable Transduction of Nondividing Cells by a Lentiviral VectorScience, 1996
- A mechanism by which adenovirus virus-associated RNAI controls translation in a transient expression assay.Molecular and Cellular Biology, 1987