Oviduct-specific expression of two therapeutic proteins in transgenic hens
- 6 February 2007
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 104 (6), 1771-1776
- https://doi.org/10.1073/pnas.0610401104
Abstract
Recent advances in avian transgenesis have led to the possibility of utilizing the laying hen as a production platform for the large-scale synthesis of pharmaceutical proteins. Ovalbumin constitutes more than half of the protein in the white of a laid egg, and expression of the ovalbumin gene is restricted to the tubular gland cells of the oviduct. Here we describe the use of lentiviral vectors to deliver transgene constructs comprising regulatory sequences from the ovalbumin gene designed to direct synthesis of associated therapeutic proteins to the oviduct. We report the generation of transgenic hens that synthesize functional recombinant pharmaceutical protein in a tightly regulated tissue-specific manner, without any evidence of transgene silencing after germ-line transmission.Keywords
This publication has 24 references indexed in Scilit:
- Estrogen action: revitalization of the chick oviduct modelTrends in Endocrinology & Metabolism, 2005
- Production of human monoclonal antibody in eggs of chimeric chickensNature Biotechnology, 2005
- Developments in transgenic technology: applications for medicineTrends in Molecular Medicine, 2005
- Efficient transgenesis in farm animals by lentiviral vectorsEMBO Reports, 2003
- Making recombinant proteins in animals – different systems, different applicationsTrends in Biotechnology, 2003
- Expression of exogenous protein in the egg white of transgenic chickensNature Biotechnology, 2002
- Germline Transmission and Tissue-Specific Expression of Transgenes Delivered by Lentiviral VectorsScience, 2002
- Production of Transgenic Quails with High Frequency of Germ-Line Transmission Using VSV-G Pseudotyped Retroviral VectorBiochemical and Biophysical Research Communications, 2001
- A far upstream estrogen response element of the ovalbumin gene contains several half-palindromic 5′-TGACC-3′ motifs acting synergisticallyCell, 1992
- Quantitation of parameters that determine the rate of ovalbumin synthesisCell, 1975