The Four Subclasses of IgG Can Be Isolated from Mouse Serum by using Protein A‐Sepharose
- 1 October 1981
- journal article
- Published by Wiley in Scandinavian Journal of Immunology
- Vol. 14 (4), 335-342
- https://doi.org/10.1111/j.1365-3083.1981.tb00573.x
Abstract
We confirmed the findings of Ey and colleagues that mouse IgG is absorbed by protein A-Sepharose at pH 8.0. Also confirmed was their finding that IgG1 mainly elutes from such a column by means of a buffer with pH 6.0 and that the corresponding pH values for IgG2a and IgG2b were 4.5 and 3.5. We made the new finding that the hulk of IgG2a bearing allotypes a or j eluted already at pH 5, in contrast to IgG2a bearing allotype b. Another newfinding was that IgG3 was mainly eluted at pH 4.5 regardless of the allotype. All four subclasses of IgG could thus be physically separated if the allotype was a or J (the only known exception is allotype b). Separation of IgG2a and IgG3 was achieved even when the allotype was b by using a pH gradient for elution. IgG2a came out al a slightly higher pH than IgG3. Mouse IgG antibodies against group A streptococcal polysaccharide belonged mostly to IgG3 and, to a lesser extent, to IgG2a and IgG2b.Keywords
This publication has 22 references indexed in Scilit:
- A Gene of the Immunoglobulin H-Chain Cluster Controls the Murine Antibody Response to Pneumococcal Polysaccharide Type 14Scandinavian Journal of Immunology, 1980
- Regulation of the anti-inulin antibody response by a nonallotype-linked gene.The Journal of Experimental Medicine, 1980
- Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-SepharoseImmunochemistry, 1978
- The natural history and immunopathology of outbred athymic (nude) miceClinical Immunology and Immunopathology, 1975
- Isolation of pure normal IgG 1 from mouse serumImmunochemistry, 1975
- Idiotypic identity of antibodies to streptococcal carbohydrate in inbred miceEuropean Journal of Immunology, 1972
- An easy method for labelling polysaccharides with radioactive iodineImmunochemistry, 1972
- Immunochemical quantitation of antigens by single radial immunodiffusionImmunochemistry, 1965
- Immunochemical quantitation of antigens by single radial immunodiffusionMolecular Immunology, 1965
- A Quantitative Immunochemical Measure of thePrimary Interaction Between I*BSA and AntibodyThe Journal of Infectious Diseases, 1958