A DNA-based method for studying root responses to drought in field-grown wheat genotypes
Open Access
- 12 November 2013
- journal article
- Published by Springer Science and Business Media LLC in Scientific Reports
- Vol. 3 (1), 3194
- https://doi.org/10.1038/srep03194
Abstract
Root systems are critical for water and nutrient acquisition by crops. Current methods measuring root biomass and length are slow and labour-intensive for studying root responses to environmental stresses in the field. Here, we report the development of a method that measures changes in the root DNA concentration in soil and detects root responses to drought in controlled environment and field trials. To allow comparison of soil DNA concentrations from different wheat genotypes, we also developed a procedure for correcting genotypic differences in the copy number of the target DNA sequence. The new method eliminates the need for separation of roots from soil and permits large-scale phenotyping of root responses to drought or other environmental and disease stresses in the field.Keywords
This publication has 39 references indexed in Scilit:
- Roots, water, and nutrient acquisition: let's get physicalTrends in Plant Science, 2012
- Extensive Pyrosequencing Reveals Frequent Intra-Genomic Variations of Internal Transcribed Spacer Regions of Nuclear Ribosomal DNAPLOS ONE, 2012
- Three-Dimensional Root Phenotyping with a Novel Imaging and Software PlatformPlant Physiology, 2011
- Root cortical aerenchyma improves the drought tolerance of maize (Zea mays L.)Plant, Cell & Environment, 2010
- Imaging and Analysis Platform for Automatic Phenotyping and Trait Ranking of Plant Root SystemsPlant Physiology, 2010
- Physical effects of soil drying on roots and crop growthJournal of Experimental Botany, 2009
- The effects of dwarfing genes on seedling root growth of wheatJournal of Experimental Botany, 2009
- Unravelling below‐ground plant distributions: a real‐time polymerase chain reaction method for quantifying species proportions in mixed root samplesMolecular Ecology Resources, 2008
- The correlation between rDNA copy number and genome size in eukaryotesGenome, 2003
- Root Architecture—Wheat as a Model PlantPublished by Informa UK Limited ,2002