Single-cell spatial reconstruction reveals global division of labour in the mammalian liver
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Open Access
- 6 February 2017
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature
- Vol. 542 (7641), 352-356
- https://doi.org/10.1038/nature21065
Abstract
Single-molecule fluorescence in situ hybridization is performed to identify several landmark genes in the liver and their level of expression in single-cell RNA sequencing is used to spatially reconstruct the zonation of all liver genes. A few important liver genes are differentially expressed in a radial fashion from the central vein outward, but the extent of such spatial division, or zonation, has remained unknown. Shalev Itzkovitz, Ido Amit and colleagues performed single-molecule fluorescence in situ hybridization against several landmark genes, and used their level of expression to spatially reconstruct the zonation of all liver genes. They find that around half of the genes in the liver are zonated, and surprisingly also find that several genes peak in the middle of the liver lobules. The succession of gene expression profiles matches the position of proteins in the bile-acid biosynthetic cascade. The mammalian liver consists of hexagon-shaped lobules that are radially polarized by blood flow and morphogens1,2,3,4. Key liver genes have been shown to be differentially expressed along the lobule axis, a phenomenon termed zonation5,6, but a detailed genome-wide reconstruction of this spatial division of labour has not been achieved. Here we measure the entire transcriptome of thousands of mouse liver cells and infer their lobule coordinates on the basis of a panel of zonated landmark genes, characterized with single-molecule fluorescence in situ hybridization7. Using this approach, we obtain the zonation profiles of all liver genes with high spatial resolution. We find that around 50% of liver genes are significantly zonated and uncover abundant non-monotonic profiles that peak at the mid-lobule layers. These include a spatial order of bile acid biosynthesis enzymes that matches their position in the enzymatic cascade. Our approach can facilitate the reconstruction of similar spatial genomic blueprints for other mammalian organs.Keywords
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