Hypersensitive Ethylene Signaling and ZMdPG1 Expression Lead to Fruit Softening and Dehiscence
Open Access
- 20 March 2013
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 8 (3), e58745
- https://doi.org/10.1371/journal.pone.0058745
Abstract
‘Taishanzaoxia’ fruit rapid softening and dehiscence during ripening stage and this process is very sensitive to endogenous ethylene. In this study, we cloned five ethylene signal transcription factors (ZMdEIL1, ZMdEIL2, ZMdEIL3, ZMdERF1 and ZMdERF2) and one functional gene, ZMdPG1, encoding polygalacturonase that could loose the cell connection which associated with fruit firmness decrease and fruit dehiscence to illustrate the reasons for this specific fruit phenotypic and physiological changes. Expression analysis showed that ZMdERF1 and ZMdEIL2 transcription were more abundant in ‘Taishanzaoxia’ softening fruit and dehiscent fruit and their expression was inhibited by an ethylene inhibitor 1-methylcyclopropene. Therefore, ZMdERF1 and ZMdEIL2 expression were responses to endogenous ethylene and associated with fruit softening and dehiscence. ZMdPG1 expression was induced when fruit softening and dehiscence but this induction can be blocked by 1-MCP, indicating that ZMdPG1 was essential for fruit softening and dehiscence and its expression was mediated by the endogenously occurred ethylene. ZMdPG1 overexpression in Arabidopsis led to silique early dehiscence while suppressing ZMdPG1 expression by antisense ZMdPG1 prevented silique naturally opening. The result also suggested that ZMdPG1 related with the connection between cells that contributed to fruit softening and dehiscence. ZMdERF1 was more closely related with ethylene signaling but it was not directly regulated the ZMdPG1, which might be regulated by the synergic pattern of ethylene transcription factors because of both the ZMdERF1 and ZMdERF2 could interact with ZMdEIL2.Keywords
This publication has 54 references indexed in Scilit:
- The Jasmonate-ZIM Domain Proteins Interact with the R2R3-MYB Transcription Factors MYB21 and MYB24 to Affect Jasmonate-Regulated Stamen Development in ArabidopsisPlant Cell, 2011
- Kiwifruit EIL and ERF Genes Involved in Regulating Fruit RipeningPlant Physiology, 2010
- Melon EIN3-like transcription factors (CmEIL1 and CmEIL2) are positive regulators of an ethylene- and ripening-induced 1-aminocyclopropane-1-carboxylic acid oxidase gene (CM-ACO1)Plant Science, 2010
- The Role of Ethylene and Cold Temperature in the Regulation of the Apple POLYGALACTURONASE1 Gene and Fruit SofteningPlant Physiology, 2010
- Ripening of fleshy fruit: Molecular insight and the role of ethyleneBiotechnology Advances, 2009
- A Versatile Zero Background T-Vector System for Gene Cloning and Functional GenomicsPlant Physiology, 2009
- ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE1 (ADPG1), ADPG2, and QUARTET2 Are Polygalacturonases Required for Cell Separation during Reproductive Development inArabidopsisPlant Cell, 2009
- Ethylene signaling: new levels of complexity and regulationCurrent Opinion in Plant Biology, 2008
- Ethylene-induced modulation of genes associated with the ethylene signalling pathway in ripening kiwifruitJournal of Experimental Botany, 2008
- Molecular biology of ethylene during tomato fruit development and maturationPlant Science, 2008