[Effects of smoking on the lipid peroxides antioxidation and alpha 1-antitrypsin].

Abstract

Detecting free radicals and alpha 1-antitrypsin (alpha 1-AT) from healthy smoker's blood to study the action of free radicals in smoking induced diseases. Thiobarbituric acid method (TBA), RIA, 5-5'-dithio-2-dinitrobenzoicacid (DTNB) method, Ellman method and enzyme linked immunosorbent assay were used to detect the lipid peroxides (LPO), superoxide dismutase (SOD), glutathione peroxidase (GSH-px), glutathione (GSH) and alpha 1-AT. In the blood of nonsmokers, LPO was 4.9 +/- 2.5 mumol/L, SOD was 878 +/- 302 ng/g Hb, GSH-px was 120 +/- 35 U, GSH was 3.4 +/- 0.7 mmol/L, alpha 1-AT was 75 +/- 11 mg/L, in the blood of smokers, they were 6.1 +/- 3.0 mumol/L, 780 +/- 282 ng/g Hb, 112 +/- 34 U, 3.1 +/- 0.8 mmol/L, 71 +/- 15 mg/L respectively. The LPO content in the blood of smokers was significant higher than that of nonsmokers (P < 0.01), but the SOD GSH-px GSH and alpha 1-AT contents measured was significant lower (P < 0.05 or P < 0.01). The increase of oxidation and decrease of antioxidation, activity of alpha 1-AT and imbalance of protease and antiprotease might play important roleg in smoking induced diseases.