Canine epitheliotropic cutaneous T‐cell lymphoma: an investigation of T‐cell receptor immunophenotype, lesion topography and molecular clonality
- 1 October 2009
- journal article
- Published by Wiley in Veterinary Dermatology
- Vol. 20 (5-6), 569-576
- https://doi.org/10.1111/j.1365-3164.2009.00814.x
Abstract
Canine epitheliotropic cutaneous T-cell lymphoma (CTCL) is a spontaneous neoplasm of the skin and mucous membranes of aged dogs. The WHO classification of tumours of haematopoietic and lymphoid tissues in human beings recognizes three forms of cutaneous epitheliotropic CTCL: mycosis fungoides (MF), Sézary syndrome and pagetoid reticulosis. In this series of dogs (n = 56), there were 39 cases of MF, 16 cases of pagetoid reticulosis and a single case of Sézary syndrome. Epitheliotropic T cells in CTCL lesions expressed CD8 in 44 of 55 dogs (80%) assessed; neither CD4 nor CD8 was expressed in the remainder. This contrasts with human MF in which alphabeta T-cell receptors (TCR) and CD4 are dominantly expressed. Molecular clonality assessment of canine epitheliotropic CTCL utilizing PCR primers specific for canine TCR gamma (TCRG) was performed. Of the 45 canine cases assessed, TCRG monoclonality was detected in 36 cases (80%). TCR typing of canine epitheliotropic CTCL revealed that TCRgammadelta was expressed in 60% of cases, including all cases of canine pagetoid reticulosis assessed. Either muco-cutaneous junctions or tissues of the oral cavity were the sites of lesions in 32 dogs (57%) with epitheliotropic CTCL. Analysis of the topography of lesions revealed an association with TCR type. If epitheliotropic CTCL lesions occurred in both locations, T cells were more likely to express TCRgammadelta (gammadelta : alphabeta = 2.0). These data establish that canine skin trafficking T cells have a far wider range than previously thought; this includes tongue, gingival, buccal and palatine mucosae.Keywords
This publication has 40 references indexed in Scilit:
- Investigation on the association between atopic dermatitis and the development of mycosis fungoides in dogs: a retrospective case-control studyVeterinary Dermatology, 2007
- CD4/CD8 double-negative epidermotropic cutaneous T-cell lymphoma: An immunohistochemical variant of mycosis fungoidesJournal of the American Academy of Dermatology, 2006
- FC-29 Distribution of gammadelta-T lymphocytes in normal canine skinVeterinary Dermatology, 2004
- Mycosis fungoides involving the oral mucosa in a childClinical and Experimental Dermatology, 2003
- Hypopigmented Mycosis FungoidesThe American Journal of Surgical Pathology, 2002
- Monoclonal antibodies that define canine homologues of human CD antigens: Summary of the First International Canine Leukocyte Antigen Workshop (CLAW)Tissue Antigens, 1994
- Correlations of unique clinical, immunotypic, and histologic findings in cutaneous gamma/delta T-cell lymphomaJournal of the American Academy of Dermatology, 1992
- Cutaneous T cell lymphoma with suppressor/cytotoxic (CD8) phenotype: Identification of rapidly progressive and chronic subtypesJournal of the American Academy of Dermatology, 1990
- A Canine Cutaneous Lymphoproliferative Disease Resembling Mycosis Fungoides in ManVeterinary Pathology, 1978
- GENERALIZED CUTANEOUS ERUPTION IN A DOG, WITH HISTOLOGICAL SIMILARITY TO HUMAN MYCOSIS FUNGOIDESBritish Journal of Dermatology, 1972