Reduced MAP Kinase Phosphatase-1 Degradation After p42/p44 MAPK -Dependent Phosphorylation
- 24 December 1999
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 286 (5449), 2514-2517
- https://doi.org/10.1126/science.286.5449.2514
Abstract
The mitogen-activated protein (MAP) kinase cascade is inactivated at the level of MAP kinase by members of the MAP kinase phosphatase (MKP) family, including MKP-1. MKP-1 was a labile protein in CCL39 hamster fibroblasts; its degradation was attenuated by inhibitors of the ubiquitin-directed proteasome complex. MKP-1 was a target in vivo and in vitro for p42 MAPK or p44 MAPK , which phosphorylates MKP-1 on two carboxyl-terminal serine residues, Serine 359 and Serine 364. This phosphorylation did not modify MKP-1's intrinsic ability to dephosphorylate p44 MAPK but led to stabilization of the protein. These results illustrate the importance of regulated protein degradation in the control of mitogenic signaling.Keywords
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