Aqueous Two-Phase Extraction Combined with Chromatography: New Strategies for Preparative Separation and Purification of Capsaicin from Capsicum Oleoresin
- 30 October 2014
- journal article
- Published by Springer Science and Business Media LLC in Applied Biochemistry and Biotechnology
- Vol. 175 (2), 1018-1034
- https://doi.org/10.1007/s12010-014-1303-6
Abstract
Capsaicin was preparatively separated and purified from capsicum oleoresin with a new method combined with aqueous two-phase extraction (ATPE) and chromatography. Screening experiments of ATPE systems containing salts and hydrophilic alcohols showed that potassium carbonate/ethanol system was the most suitable system for capsaicin recovery among the systems considered. Response surface methodology was used to determine an optimized aqueous two-phase system for the extraction of capsaicin from capsaicin oleoresin. In a 20 % (w/w) ethanol/22.3 % (w/w) potassium carbonate system, 85.4 % of the capsaicin was recovered in the top ethanol-rich phase while most oil and capsanthin ester were removed in the interphase. The capsaicinoid extract was then subjected to two chromatographic steps using D101 macroporous resin and inexpensive SKP-10-4300 reverse-phase resin first applied for the purification of capsaicin. After simple optimization of loading/elution conditions for D101 macroporous resin chromatography and SKP-10-4300 reverse-phase resin chromatography, the purities of capsaicin were improved from 7 to 85 %. In the two chromatography processes, the recoveries of capsaicin were 93 and 80 % respectively; the productivities of capsaicin were 1.86 and 4.2 (g capsaicin/L resin) per day respectively. It is worth mentioning that a by-product of capsaicin production was also obtained with a high purity (90 %).Keywords
This publication has 32 references indexed in Scilit:
- Chromatographic Separation and Characterization of Capsaicinoids and Their AnaloguesPharmacology & Pharmacy, 2011
- Preparative enrichment and separation of astragalosides from Radix Astragali extracts using macroporous resinsJournal of Separation Science, 2010
- Preparative separation of capsaicin and dihydrocapsaicin from Capsicum frutescens by high‐speed counter‐current chromatographyJournal of Separation Science, 2009
- Recovery of Polyphenolics from Apple Juice Utilizing Adsorbent Polymer TechnologyInternational Journal of Food Engineering, 2008
- The emerging role of TRPV1 in diabetes and obesityTrends in Pharmacological Sciences, 2008
- Recovery of benzaldehyde from aqueous streams using extractant impregnated resinsReactive and Functional Polymers, 2006
- Molecular Dynamics Study of the Properties of Capsaicin in an 1-Octanol/Water SystemThe Journal of Physical Chemistry B, 2006
- Investigation of extractive citric acid fermentation using response-surface methodologyProcess Biochemistry, 2002
- Protein Purification via Aqueous Two‐Phase Extraction (ATPE) and Immobilized Metal Affinity Chromatography. Effectiveness of Salt Addition To Enhance Selectivity and Yield of GFPuvBiotechnology Progress, 2002
- Improved high-performance liquid chromatography method for the determination of major capsaicinoids in Capsicum oleoresinsJournal of Agricultural and Food Chemistry, 1991