Imipenem-EDTA Disk Method for Differentiation of Metallo-β-Lactamase-Producing Clinical Isolates of Pseudomonas spp. and Acinetobacter spp
Open Access
- 1 October 2002
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 40 (10), 3798-3801
- https://doi.org/10.1128/jcm.40.10.3798-3801.2002
Abstract
Rapid detection of metallo-β-lactamase (MBL)-producing gram-negative bacilli is necessary to prevent their dissemination. The method using a disk with imipenem plus 750 μg of EDTA differentiated all MBL-producing pseudomonads, and the sensitivity and specificity for acinetobacters were 95.7 and 91.0%, respectively. The imipenem-EDTA disks were stable for 12 and 16 weeks at 4 and −20°C, respectively.Keywords
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