Synapsin I is a spectrin-binding protein immunologically related to erythrocyte protein 4.1
- 1 May 1985
- journal article
- Published by Springer Science and Business Media LLC in Nature
- Vol. 315 (6018), 410-413
- https://doi.org/10.1038/315410a0
Abstract
The membrane-associated cytoskeleton is considered to be the apparatus by which cells regulate the properties of their plasma membranes, although recent evidence has indicated additional roles for the proteins of this structure, including an involvement in intracellular transport and exocytosis (see refs 1-3 for review). Of the membrane skeletal proteins, to date only spectrin (fodrin) and ankyrin have been purified and characterized from non-erythroid sources. Protein 4.1 in the red cell is a spectrin-binding protein that enhances the binding of spectrin to actin and can apparently bind to at least one transmembrane protein Immunoreactive forms of 4.1 have been detected in several cell types, including brain. Here we report the purification of brain 4.1 on the basis of its cross-reactivity with erythrocyte 4.1 and spectrin-binding activity. We further show that brain 4.1 is identical to the synaptic vesicle protein, synapsin I, one of the brain's major substrates for cyclic AMP and Ca2+-calmodulin-dependent kinases. Spectrin and synapsin are present in brain homogenates in an approximately 1:1 molar ratio. Although synapsin I has been implicated in synaptic transmission, no activity has been previously ascribed to it.Keywords
This publication has 30 references indexed in Scilit:
- Cell biology: A spectrum of spectrinsNature, 1984
- Functional characterization of human erythrocyte spectrin .alpha. and .beta. chains: association with actin and erythrocyte protein 4.1Biochemistry, 1984
- Analysis of the ternary interaction of the red cell membrane skeletal proteins, spectrin, actin, and 4.1Biochemistry, 1984
- An analogue of the erythroid membrane skeletal protein 4.1 in nonerythroid cells.The Journal of cell biology, 1984
- Membrane skeletal protein 4.1 of avian erythrocytes is composed of multiple variants that exhibit tissue-specific expressionCell, 1984
- Identification and Location of Brain Protein 4.1Science, 1984
- Glycophorin is linked by band 4.1 protein to the human erythrocyte membrane skeletonNature, 1984
- Cell membrane skeleton: The spread of spectrinNature, 1983
- A protein immunologically related to erythrocyte band 4.1 is found on stress fibres of non-erythroid cellsNature, 1982
- In vitro formation of a complex between cytoskeletal proteins of the human erythrocyteNature, 1979