Vibrio cholerae non‐O1, non‐O139 strains isolated before 1992 from Varanasi, India are multiple drug resistant, contain intSXT, dfr18 and aadA5 genes
- 30 January 2008
- journal article
- Published by Wiley in Environmental Microbiology
- Vol. 10 (4), 866-873
- https://doi.org/10.1111/j.1462-2920.2007.01502.x
Abstract
In this study, we report the presence of the SXT element and Class I integron in Vibrio cholerae non-O1, non-O139 strains isolated from Varanasi, India. Isolates were resistant to cotrimoxazole, trimethoprim and/or streptomycin, furazolidone and ampicillin. None contained plasmids. Polymerase chain reaction (PCR) and DNA sequencing revealed the presence of antibiotic resistance gene cassettes, aadA1, aadA2, aadA5 and dfrA15, in the Class I integron and SXT, an integrative conjugative element containing dfr18, sulII and strAB, in three and six of the isolates respectively. Conjugation experiments, followed by PCR analysis of transconjugants, provided evidence for the transferable nature of intSXT and associated antibiotic resistance gene cassettes. This is the first report of the occurrence of SXT ICE, dfr18, sulII, strAB and aadA5 genes in environmental V. cholerae non-O1, non-O139 strains from Varanasi, India, that had been isolated before 1992.Keywords
This publication has 30 references indexed in Scilit:
- Multiplex PCR for detection of antibiotic resistance genes and the SXT element: application in the characterization of Vibrio choleraeJournal of Medical Microbiology, 2007
- Prevalence of aadA1 and dfrA15 class 1 integron cassettes and SXT circulation in Vibrio cholerae O1 isolates from AfricaJournal of Antimicrobial Chemotherapy, 2006
- Distribution and characterization of integrons in various serogroups of Vibrio cholerae strains isolated from diarrhoeal patients between 1992 and 2000 in Kolkata, IndiaJournal of Medical Microbiology, 2006
- SXT-Related Integrating Conjugative Element in New World Vibrio choleraeApplied and Environmental Microbiology, 2006
- Antibiotic Resistance Conferred by a Class I Integron and SXT Constin in Vibrio cholerae O1 Strains Isolated in LaosAntimicrobial Agents and Chemotherapy, 2004
- Molecular Characterization of Vibrio cholerae O139 Bengal Isolated from Water and the Aquatic Plant Eichhornia crassipes in the River Ganga, Varanasi, IndiaApplied and Environmental Microbiology, 2003
- Occurrence of Antibiotic Resistance Gene Cassettes aac ( 6 ′) -Ib , dfrA5 , dfrA12 , and ereA2 in Class I Integrons in Non-O1, Non-O139 Vibrio cholerae Strains in IndiaAntimicrobial Agents and Chemotherapy, 2002
- Genomic and Functional Analyses of SXT, an Integrating Antibiotic Resistance Gene Transfer Element Derived from Vibrio choleraeJournal of Bacteriology, 2002
- Distribution and Content of Class 1 Integrons in Different Vibrio cholerae O-Serotype Strains Isolated in ThailandAntimicrobial Agents and Chemotherapy, 2000
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975